Gut microbiota and metabolites of α-synuclein transgenic monkey models with early stage of Parkinson’s disease

• Published in: NPJ biofilms and microbiomes 2021-09, Vol.7 (1), p.69-69, Article 69
• Main Authors: Yan, Yaping, Ren, Shuchao, Duan, Yanchao, Lu, Chenyu, Niu, Yuyu, Wang, Zhengbo, Inglis, Briauna, Ji, Weizhi, Zheng, Yun, Si, Wei
• Format: Article
• Language: English
• Subjects: 631/326/41/2142; 692/698/2741/2135; Acids; alpha-Synuclein - genetics; alpha-Synuclein - metabolism; Animals; Animals, Genetically Modified; Aspartic acid; Biomedical and Life Sciences; Cognitive ability; Digestive system; Disease Models, Animal; Female; Gas chromatography; Gastrointestinal Microbiome - physiology; Gastrointestinal tract; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; Humans; Intestinal microflora; Intestine; Life Sciences; Macaca mulatta; Male; Medical Microbiology; Metabolic pathways; Metabolites; Metagenomics; Mice; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microbiota; Mitochondria; Movement disorders; Neurodegenerative Diseases; Oxidative phosphorylation; p-Hydroxyphenylacetic acid; Parkinson Disease - metabolism; Parkinson's disease; Phosphorylation; Synuclein
• ISSN: 2055-5008; 2055-5008
• DOI: 10.1038/s41522-021-00242-3

Parkinson’s disease (PD) is the second most prevalent neurodegenerative disease. However, it is unclear whether microbiota and metabolites have demonstrated changes at early PD due to the difficulties in diagnosis and identification of early PD in clinical practice. In a previous study, we generated A53T transgenic monkeys with early Parkinson’s symptoms, including anxiety and cognitive impairment. Here we analyzed the gut microbiota by metagenomic sequencing and metabolites by targeted gas chromatography. The gut microbiota analysis showed that the A53T monkeys have higher degree of diversity in gut microbiota with significantly elevated Sybergistetes, Akkermansia, and Eggerthella lenta compared with control monkeys. Prevotella significantly decreased in A53T transgenic monkeys. Glyceric acid, L-Aspartic acid, and p-Hydroxyphenylacetic acid were significantly elevated, whereas Myristic acid and 3-Methylindole were significantly decreased in A53T monkeys. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (KO0131) and the oxidative phosphorylation reaction (KO2147) were significantly increased in metabolic pathways of A53T monkeys. Our study suggested that the transgenic A53T and α-syn aggregation may affect the intestine microbiota and metabolites of rhesus monkeys, and the identified five compositional different metabolites that are mainly associated with mitochondrial dysfunction may be related to the pathogenesis of PD.