Overexpression of microRNA-423-3p indicates poor prognosis and promotes cell proliferation, migration, and invasion of lung cancer

• Published in: Diagnostic pathology 2019-06, Vol.14 (1), p.53-53, Article 53
• Main Authors: Wang, Rukun, Li, Gaofeng, Zhuang, Guoyan, Sun, Shuying, Song, Zhihui
• Format: Article
• Language: English
• Subjects: Biochemistry; Bioindicators; Biomarkers; Cancer cells; Cancer diagnosis; Cancer metastasis; Cancer research; Care and treatment; Cell adhesion & migration; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement; Cell Proliferation; Cholecystokinin; Development and progression; Female; Gene expression; Gene Expression Regulation, Neoplastic - genetics; Genetic aspects; Health aspects; Humans; Invasion; Kaplan-Meier Estimate; Liver cancer; Lung cancer; Lung Neoplasms - diagnosis; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Lymph nodes; Lymphatic Metastasis; Male; Medical prognosis; Medical research; Metastases; MicroRNA; microRNA-423-3p; MicroRNAs; MicroRNAs - genetics; Middle Aged; Migration; miRNA; Mortality; Multivariate Analysis; Neoplasm Invasiveness; Physiology; Polymerase chain reaction; Prognosis; Proliferation; Regression analysis; Reverse transcription; Ribonucleic acid; RNA; Statistical analysis; Surgery; Therapeutic applications; Tumor cell lines; Tumors; Up-Regulation; Variance analysis
• ISSN: 1746-1596; 1746-1596
• DOI: 10.1186/s13000-019-0831-3

Lung cancer is one of the common malignant tumors worldwide with high incidence and mortality. MicroRNA-423-3p (miR-423-3p) acts as an oncogene in several types of cancers. The aim of this study is to reveal the clinical significance and biological function of miR-423-3p in lung cancer. The expression of miR-423-3p was detected in lung cancer specimens by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were used to investigate the prognostic significance of miR-423-3p in lung cancer. CCK-8 and Transwell assays were used to determine the functional role of miR-423-3p in lung cancer. We observed that miR-423-3p was significantly upregulated in lung cancer tissues and cell lines. Overexpression of miR-423-3p was significantly associated with lymph node metastasis, TNM stage, and poor prognosis. Multivariate Cox regression analysis results showed that miR-423-3p was an independent prognostic indicator for lung cancer patients. Results of functional analyses revealed that overexpression of miR-423-3p promoted cell proliferation, migration, and invasion in lung cancer cells. These results indicated that miR-423-3p acts as an oncogene and promotes cell proliferation migration, and invasion of lung cancer. And miR-423-3p may serve as a potential prognostic biomarker and therapeutic target for the treatment of lung cancer.