Development of a membrane for guided tissue regeneration: An in vitro study

• Published in: Indian journal of dental research 2020-09, Vol.31 (5), p.763-767
• Main Authors: Fernandes, Ramon, Damasceno, MáRio, Pimentel, Gabriel, Mendonça, Juliano, Gelfuso, Maria, Pereira, SéRgio Da Silva, Passos, Vanara
• Format: Article
• Language: English
• Subjects: Chitosan; guided tissue regeneration; Light microscopy; membranes; Nylon; Physical properties; Statistical analysis; Statistics; Tetrafluoroethylene; Tissue engineering
• ISSN: 0970-9290; 1998-3603
• DOI: 10.4103/ijdr.IJDR_244_19

Aim: The aim of this study was to develop an alternative low-cost membrane for use in guided tissue regeneration (GTR). Setting and Design: In vitro study. Methods and Material: In this study, a membrane prepared from a 335 mm sized opening nylon substrate, covered in aqueous resin derived from chitosan, was compared with a commercial material, a non-degradable expanded poly (tetrafluoroethylene). Nylon substrate samples 2.0 × 2.0 cm were covered by aqueous resin based on diluted chitosan solution into 1:05 or 1:10 by spin coating technique to produce from 06, 10, and 15 layers. The surfaces of these membranes were observed using optical microscopy. The physical properties were measured by hydration superficial energy measurements (ΔG) and a tensile test machine. Statistical Analysis: Statistical analysis was performed using the Student's t test at a significance level of 5%, using the BioEstat 2.0 program. Results: The Δ G values of the nylon membrane covered by the 1:05 of chitosan with 15 layers were close to the commercial membrane's Δ G values. The tensile strength values of the nylon membrane covered by the 1:05 of chitosan with 15 layers were higher than the commercial membrane's (115.826 MPa, P < 0.05). Conclusion: Therefore, the membrane developed shows some favorable physical properties that could qualify it as a material candidate for use in guided tissue regeneration.