Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication

Porcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag prot...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-06, Vol.21 (12), p.4386
Main Authors: Zhao, Tingting, Cui, Li, Yu, Xiangqian, Zhang, Zhonghai, Chen, Qi, Hua, Xiuguo
Format: Article
Language:English
Subjects:
Animals
Biomedical materials
Cell adhesion & migration
Cell Line
Enteritis
Gene Expression Regulation
Genes
Infections
Mass spectrometry
Models, Biological
Osteopontin
Pathogenesis
Peptides
Picornaviridae - physiology
Picornaviridae Infections - genetics
Picornaviridae Infections - metabolism
Picornaviridae Infections - veterinary
Polioencephalomyelitis
Protein biosynthesis
Proteins
Proteomes
Proteomics
Proteomics - methods
quantitative proteomics
Replication
Reproductive disorders
RNA viruses
sapelovirus
Scientific imaging
Swine
Swine Diseases - genetics
Swine Diseases - metabolism
Swine Diseases - virology
Syndecan
Syndecan-1 - genetics
Syndecan-1 - metabolism
Tandem Mass Spectrometry
tandem mass tag
Ubiquitin
Vinculin
Viral infections
Viral Load
Viral Proteins - metabolism
Virus Replication
Viruses
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Summary:Porcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21124386