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Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections
Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MR...
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| Published in: | Iranian journal of microbiology 2024-08, Vol.16 (4), p.443-449 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 449 |
| container_issue | 4 |
| container_start_page | 443 |
| container_title | Iranian journal of microbiology |
| container_volume | 16 |
| creator | Krishnakumar, Sharanya Khalid, Abdul Azeez Mohamed Sowndarya, Jothipandian Krishnasamy, Lakshmi Nithyanand, Paramasivam |
| description | Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MRSA strains by phenotypic and genotypic methods and to analyze the antibiotic susceptibility pattern of MRSA strains from patients attending a tertiary care hospital.
This study was conducted over a period of 1 year, where 296 isolates of
were isolated from various clinical specimens. The isolated strains were examined for antibiotic susceptibility by the modified Kirby Bauer disc diffusion method. Methicillin resistance was detected by cefoxitin disk diffusion test.
A total of 104 isolates were found to be MRSA and 192 were found to be MSSA. Among the 104 MRSA isolates, 10 strains that were multidrug resistant were subjected to 16S rRNA gene sequencing analysis. All the 10 strains had a 99% match with
strains that were responsible for causing some serious biofilm mediated clinical manifestations like cystic fibrosis and device mediated infections. The biofilms were quantified using crystal violet staining and their ability to produce biofilms was analyzed using scanning electron microscopy and matched with the Genbank.
Hence these phylogenetic analysis aid in treating the patients and combating resistance to antibiotics. |
| doi_str_mv | 10.18502/ijm.v16i4.16302 |
| format | article |
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This study was conducted over a period of 1 year, where 296 isolates of
were isolated from various clinical specimens. The isolated strains were examined for antibiotic susceptibility by the modified Kirby Bauer disc diffusion method. Methicillin resistance was detected by cefoxitin disk diffusion test.
A total of 104 isolates were found to be MRSA and 192 were found to be MSSA. Among the 104 MRSA isolates, 10 strains that were multidrug resistant were subjected to 16S rRNA gene sequencing analysis. All the 10 strains had a 99% match with
strains that were responsible for causing some serious biofilm mediated clinical manifestations like cystic fibrosis and device mediated infections. The biofilms were quantified using crystal violet staining and their ability to produce biofilms was analyzed using scanning electron microscopy and matched with the Genbank.
Hence these phylogenetic analysis aid in treating the patients and combating resistance to antibiotics.</description><identifier>ISSN: 2008-3289</identifier><identifier>EISSN: 2008-4447</identifier><identifier>DOI: 10.18502/ijm.v16i4.16302</identifier><identifier>PMID: 39267936</identifier><language>eng</language><publisher>Iran: Tehran University of Medical Sciences</publisher><subject>Biofilm ; Methicillin-resistant Staphylococcus aureus ; Multi drug resistance ; Original ; Penicillin binding proteins ; Pyogenic infection ; Staphylococcus aureus</subject><ispartof>Iranian journal of microbiology, 2024-08, Vol.16 (4), p.443-449</ispartof><rights>Copyright© 2024 The Authors. Published by Tehran University of Medical Sciences.</rights><rights>Copyright© 2024 The Authors. Published by Tehran University of Medical Sciences. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389768/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389768/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39267936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krishnakumar, Sharanya</creatorcontrib><creatorcontrib>Khalid, Abdul Azeez Mohamed</creatorcontrib><creatorcontrib>Sowndarya, Jothipandian</creatorcontrib><creatorcontrib>Krishnasamy, Lakshmi</creatorcontrib><creatorcontrib>Nithyanand, Paramasivam</creatorcontrib><title>Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections</title><title>Iranian journal of microbiology</title><addtitle>Iran J Microbiol</addtitle><description>Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MRSA strains by phenotypic and genotypic methods and to analyze the antibiotic susceptibility pattern of MRSA strains from patients attending a tertiary care hospital.
This study was conducted over a period of 1 year, where 296 isolates of
were isolated from various clinical specimens. The isolated strains were examined for antibiotic susceptibility by the modified Kirby Bauer disc diffusion method. Methicillin resistance was detected by cefoxitin disk diffusion test.
A total of 104 isolates were found to be MRSA and 192 were found to be MSSA. Among the 104 MRSA isolates, 10 strains that were multidrug resistant were subjected to 16S rRNA gene sequencing analysis. All the 10 strains had a 99% match with
strains that were responsible for causing some serious biofilm mediated clinical manifestations like cystic fibrosis and device mediated infections. The biofilms were quantified using crystal violet staining and their ability to produce biofilms was analyzed using scanning electron microscopy and matched with the Genbank.
Hence these phylogenetic analysis aid in treating the patients and combating resistance to antibiotics.</description><subject>Biofilm</subject><subject>Methicillin-resistant Staphylococcus aureus</subject><subject>Multi drug resistance</subject><subject>Original</subject><subject>Penicillin binding proteins</subject><subject>Pyogenic infection</subject><subject>Staphylococcus aureus</subject><issn>2008-3289</issn><issn>2008-4447</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkk1v1DAQhiMEolXpnRPKkcsu_o59QqgCWqkSSMDZsp3xxqskDrbTahE_vtkPVtSX8YznfWYkv1X1FqM1lhyRD2E7rB-wCGyNBUXkRXVJEJIrxljz8nSnRKqL6jrnLVrOkgnBX1cXVBHRKCouq7_fOxhj2U3B1WZs6805c51JxhVI4Y8pIY519PUApQsu9H0Y6wQ55GLGUv8oZup2fXTRuTnXZk6wDzlHF0yBtn4MpaunXVzgCziMHtyemN9Ur7zpM1yf4lX168vnnze3q_tvX-9uPt2vHBGYrMBbj4lvHQEmnedtSwXj0hPmSGOQdcwqxrFV3EoGnnJFGwFgiZIYtYLSq-ruyG2j2eophcGknY4m6EMhpo02qQTXg264ErhBDBvFmePSLqO9FYZa1kjm_ML6eGRNsx2gdTCWZPpn0OcvY-j0Jj5ojKlUjZAL4f2JkOLvGXLRQ8gO-t6MEOesKUaME0XovhUdW12KOSfw5zkY6YMJ9GICfTCBPphgkbz7f7-z4N-X0yd_vbL9</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Krishnakumar, Sharanya</creator><creator>Khalid, Abdul Azeez Mohamed</creator><creator>Sowndarya, Jothipandian</creator><creator>Krishnasamy, Lakshmi</creator><creator>Nithyanand, Paramasivam</creator><general>Tehran University of Medical Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20240801</creationdate><title>Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections</title><author>Krishnakumar, Sharanya ; Khalid, Abdul Azeez Mohamed ; Sowndarya, Jothipandian ; Krishnasamy, Lakshmi ; Nithyanand, Paramasivam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2612-efbf12fdc2e48cf5dd36458f24c27a0bc4b9451b95b84ef359376eeb29810d633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biofilm</topic><topic>Methicillin-resistant Staphylococcus aureus</topic><topic>Multi drug resistance</topic><topic>Original</topic><topic>Penicillin binding proteins</topic><topic>Pyogenic infection</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krishnakumar, Sharanya</creatorcontrib><creatorcontrib>Khalid, Abdul Azeez Mohamed</creatorcontrib><creatorcontrib>Sowndarya, Jothipandian</creatorcontrib><creatorcontrib>Krishnasamy, Lakshmi</creatorcontrib><creatorcontrib>Nithyanand, Paramasivam</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Iranian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krishnakumar, Sharanya</au><au>Khalid, Abdul Azeez Mohamed</au><au>Sowndarya, Jothipandian</au><au>Krishnasamy, Lakshmi</au><au>Nithyanand, Paramasivam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections</atitle><jtitle>Iranian journal of microbiology</jtitle><addtitle>Iran J Microbiol</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>16</volume><issue>4</issue><spage>443</spage><epage>449</epage><pages>443-449</pages><issn>2008-3289</issn><eissn>2008-4447</eissn><abstract>Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MRSA strains by phenotypic and genotypic methods and to analyze the antibiotic susceptibility pattern of MRSA strains from patients attending a tertiary care hospital.
This study was conducted over a period of 1 year, where 296 isolates of
were isolated from various clinical specimens. The isolated strains were examined for antibiotic susceptibility by the modified Kirby Bauer disc diffusion method. Methicillin resistance was detected by cefoxitin disk diffusion test.
A total of 104 isolates were found to be MRSA and 192 were found to be MSSA. Among the 104 MRSA isolates, 10 strains that were multidrug resistant were subjected to 16S rRNA gene sequencing analysis. All the 10 strains had a 99% match with
strains that were responsible for causing some serious biofilm mediated clinical manifestations like cystic fibrosis and device mediated infections. The biofilms were quantified using crystal violet staining and their ability to produce biofilms was analyzed using scanning electron microscopy and matched with the Genbank.
Hence these phylogenetic analysis aid in treating the patients and combating resistance to antibiotics.</abstract><cop>Iran</cop><pub>Tehran University of Medical Sciences</pub><pmid>39267936</pmid><doi>10.18502/ijm.v16i4.16302</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2008-3289 |
| ispartof | Iranian journal of microbiology, 2024-08, Vol.16 (4), p.443-449 |
| issn | 2008-3289 2008-4447 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_759617041a954c58bbf1fb6a3b4784cf |
| source | PMC (PubMed Central); DOAJ Directory of Open Access Journals |
| subjects | Biofilm Methicillin-resistant Staphylococcus aureus Multi drug resistance Original Penicillin binding proteins Pyogenic infection Staphylococcus aureus |
| title | Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections |
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