A Longitudinal Evaluation of the Bacterial Pathogens Colonizing Chronic Non-Healing Wound Sites at a United States Military Treatment Facility in the Pacific Region

• Published in: Infection and drug resistance 2021-01, Vol.14, p.1-10
• Main Authors: Nahid, Md A, Griffin, Jaclyn M, Lustik, Michael B, Hayes, Jordan J, Fong, Keith S K, Horseman, Timothy S, Menguito, Massimo, Snesrud, Erik C, Barnhill, Jason C, Washington, Michael A
• Format: Article
• Language: English
• Subjects: Analysis; anti-microbial resistance; Antibiotic resistance; Antibiotics; Antimicrobial agents; Antimicrobial resistance; antimicrobial susceptibility profiling; Bacteria; Biotechnology industry; Care and treatment; Drug resistance; Drug resistance in microorganisms; Genomes; Genomics; Gram-positive bacteria; Human subjects; Laboratories; Medical equipment and supplies industry; Medical test kit industry; Methicillin; Microbiota (Symbiotic organisms); Microflora; non-healing wound; Original Research; Pathogenic microorganisms; Pathogens; Patients; Staphylococcus aureus; Strains (organisms); Surveillance; Ulcers; Whole genome sequencing; Wound healing; wound microflora; Wounds and injuries
• ISSN: 1178-6973; 1178-6973
• DOI: 10.2147/IDR.S260708

The biology of chronic wounds is complex and many factors act concurrently to impede healing progress. In this study, the dynamics of microflora changes and their antibiotic susceptibility patterns were evaluated longitudinally over 30 days using data from 28 patients with a total of 47 chronic lower extremity wounds. In this study, colonized wound isolates were characterized using cultural, biochemical, and VITEK 2 methods. Antibiotic susceptibility patterns of the wound isolates were analyzed using various phenotypic assays. Furthermore, antimicrobial resistance patterns and the presence of mutations were evaluated by a genotypic assay, whole-genome sequencing (WGS). and were found to be the most common strains at early time points, while members of were prevalent at later stages of infection. Antimicrobial resistance testing and whole-genome sequencing revealed that the molecular and phenotypic characteristics of the identified wound pathogens remained relatively stable throughout the study period. It was also noted that and species may serve as reservoirs for quinolone resistance in the Pacific region. Our observations showed that wounds were colonized with diverse bacteria and interestingly their numbers and/or types were changed over the course of infection. The rapid genetic changes that accompanied the first 4 weeks after presentation did not directly contribute to the development of antibiotic resistance. In addition, standard wound care procedures did not appear to select for resistant bacterial strains. Future efforts should focus on defining those genetic changes associated with the wound colonizing microorganisms that occur beyond 4 weeks.