Evodiamine Inhibits Helicobacter pylori Growth and Helicobacter pylori -Induced Inflammation

• Published in: International journal of molecular sciences 2021-03, Vol.22 (7), p.3385
• Main Authors: Yang, Ji Yeong, Kim, Jong-Bae, Lee, Pyeongjae, Kim, Sa-Hyun
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Antiinfectives and antibacterials; Bacteria; Carcinogens; Clinical isolates; Cytokines; Evodia rutaecarpa; evodiamine; Gastric cancer; Gastritis; Helicobacter pylori; IL-8; Infections; Inflammation; Kinases; Lymphocytes B; MAP kinase; natural compound; NF-κB protein; Protein A; Protein kinase; Proteins; RNA polymerase; Transcription; Urease; Virulence
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22073385

( ) classified as a class I carcinogen by the World Health Organization (WHO) plays an important role in the progression of chronic gastritis and the development of gastric cancer. A major bioactive component of , evodiamine, has been known for its anti-bacterial effect and anti-cancer effects. However, the inhibitory effect of evodiamine against is not yet known and the inhibitory mechanisms of evodiamine against gastric cancer cells are yet to be elucidated concretely. In this study, therefore, anti-bacterial effect of evodiamine on growth and its inhibitory mechanisms as well as anti-inflammatory effects and its mechanisms of evodiamine on -induced inflammation were investigated in vitr. Results of this study showed the growth of the reference strains and clinical isolates were inhibited by evodiamine. It was considered one of the inhibitory mechanisms that evodiamine downregulated both gene expressions of replication and transcription machineries of . Treatment of evodiamine also induced downregulation of urease and diminished translocation of cytotoxin-associated antigen A (CagA) and vacuolating cytotoxin A (VacA) proteins into gastric adenocarcinoma (AGS) cells. This may be resulted from the reduction of CagA and VacA expressions as well as the type IV secretion system (T4SS) components and secretion system subunit protein A (SecA) protein which are involved in translocation of CagA and VacA into host cells, respectively. In particular, evodiamine inhibited the activation of signaling proteins such as the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) and the mitogen-activated protein kinase (MAPK) pathway induced by infection. It consequently might contribute to reduction of interleukin (IL)-8 production in AGS cells. Collectively, these results suggest anti-bacterial and anti-inflammatory effects of evodiamine against .