Distribution of 22 cytokine gene polymorphisms in Roma from the Republic of Macedonia

The aim of this study was to analyze 22 cytokine polymorphisms in the Roma population from the Republic of Macedonia. The Roma population consists of 77 healthy unrelated individuals, residents of different geographical regions of the Republic of Macedonia (Skopje, Gostivar, and Kochani). Blood samp...

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Published in:Iranian journal of allergy, asthma, and immunology asthma, and immunology, 2012-12, Vol.11 (4), p.282-293
Main Authors: Trajkov, Dejan, Petlichkovski, Aleksandar, Efinska-Mladenovska, Olivija, Hristomanova, Slavica, Djulejic, Eli, Kirijas, Meri, Senev, Aleksandar, Spiroski, Mirko
Format: Article
Language:English
Subjects:
Abolition of slavery
Adult
Chi-Square Distribution
Cytokine
Cytokines
Cytokines - genetics
Deoxyribonucleic acid
Disease
DNA
Female
Gene banks
Gene Frequency
Genetic diversity
Genetic polymorphism
Genetic testing
Haplotypes
Humans
Linkage Disequilibrium
Macedonia (Republic) - epidemiology
Male
Mitochondrial DNA
Models, Statistical
Polymorphism
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Roma - genetics
Romani people
Statistical methods
Studies
Young Adult
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Summary:The aim of this study was to analyze 22 cytokine polymorphisms in the Roma population from the Republic of Macedonia. The Roma population consists of 77 healthy unrelated individuals, residents of different geographical regions of the Republic of Macedonia (Skopje, Gostivar, and Kochani). Blood samples were collected after obtaining written consent. DNA was isolated from peripheral blood and 22 polymorphisms were typed: IL1A -889, IL1B -511, IL1B +3962, IL1R pst1 1970, IL1RN mspa11100, IL4RA +1902, IL12 -1188, IFNG utr5644, TGF-β1 cdn10, TGF-β1 cdn25, TNF-α -308, TNF-α -238, IL-2 -330, IL-2 +166, IL-4 -1098, IL-4 -590, IL-4 -33, IL-6 -174, IL-6 565, IL-10 -1082, IL-10 -819, and IL-10 -592. Cytokine genotyping was performed by PCR-SSP. The population genetics analysis package, PyPop, was used for analysis of the cytokine data. Fnd was negative and significantly different from 0 for IL-4 -590 (p of F=0.006), IL-10 -1082 (p of F=0.010), IFN utr5644 (p of F=0.024), IL-4 -1098 (p of F=0.026) and TGF-1 cdn25 (p of F=0.001) alleles, as well as for IL-2 haplotypes (p=0.025). Several SNPs (IL-12B -1188, IL-2 -330, IL-4 -1098, IL-4 -590, and IL-10 -1082) were not in HWP (p
ISSN:1735-1502
1735-5249