Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells

AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. ·METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. P...

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Bibliographic Details
Published in:International journal of ophthalmology 2013, Vol.6 (3), p.286-294
Main Authors: Yan, Lu-Bin, Shi, Kai, Bing, Zhi-Tong, Sun, Yi-Lan, Shen, Yang
Format: Article
Language:English
Subjects:
2D-LC-MS/MS
acids
amino
analysis
Basic Research
cell
interaction
irradiation
isotope
labeling
melanoma
melanoma cell
protein-protein
protein-protein interaction
proteomic
proteomic analysis
stable
stable isotope labeling with amino acids
with
X-ray
X-ray irradiation
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Summary:AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. ·METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein - protein interaction was used to analyze relationships among proteins. · RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. · CONCLUSION: Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.
ISSN:2222-3959
2227-4898
DOI:10.3980/j.issn.2222-3959.2013.03.06