Circular RNA Profiling Reveals That circRNA_104433 Regulates Cell Growth by Targeting miR-497-5p in Gastric Cancer

The role and mechanism of hsa_circRNA_104433 in gastric cancer (GC) are further elucidated. CircRNA_104433 was selected by circRNA microarrays and GEO database. qRT-PCR was used to analyze the expression of circRNA_104433 in GC. The role of circRNA_104433 in GC cells was evaluated based on cell cycl...

Full description

Saved in:
Bibliographic Details
Published in:Cancer management and research 2020-01, Vol.12, p.15-30
Main Authors: Wei, Weiyuan, Mo, Xianwei, Yan, Linhai, Huang, Mingwei, Yang, Yang, Jin, Qinwen, Zhong, Huage, Cao, Wenlong, Wu, Kun, Wu, Liucheng, Li, Zhao, Wang, Tingan, Qin, Yuzhou, Chen, Jiansi
Format: Article
Language:English
Subjects:
Apoptosis
Cancer
cdc25a
Cell cycle
Cell division
Cell growth
cell proliferation
circrna_104433
Criminal investigation
Cyclin-dependent kinases
Development and progression
Gastric cancer
Gene expression
Kinases
Luciferase
Medical prognosis
Medical research
MicroRNAs
mir-497-5p
Original Research
Prognosis
RNA
Stomach cancer
Studies
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The role and mechanism of hsa_circRNA_104433 in gastric cancer (GC) are further elucidated. CircRNA_104433 was selected by circRNA microarrays and GEO database. qRT-PCR was used to analyze the expression of circRNA_104433 in GC. The role of circRNA_104433 in GC cells was evaluated based on cell cycle progression, cell proliferation, cell apoptosis, and tumor xenograft experiment assay. To explore the mechanisms of circRNA_104433 in GC TCGA database, STRING version, qRT-PCR and luciferase assay were performed. Furthermore, the prognostic value of CDC25A in GC was determined based on the GEO database. The level of circRNA_104433 showed upregulation in GC tissues, and the expression of it showed a positive correlation with the degree of differentiation and the size of the tumor. Knockdown of circRNA_104433 inhibited cell cycle transition, and cell proliferation, while promoted cell apoptosis in GC. CircRNA_104433 directly binds to miR-497-5p, which directly regulates CDC25A. The median survival period of GC patients with high expression levels of CDC25A was 21.3 months, which was shorter than those with low group expression of CDC25A (35.9 months). The cell cycle proteins CDK1, CDK2, CCNB1, PKMYT1, CDC20, CHEK1 and CDC25A were coexpressed with CDC25A. These findings suggested that knockdown of circRNA_104433 expression suppressed tumor development in GC.
ISSN:1179-1322
1179-1322
DOI:10.2147/CMAR.S219307