Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement

The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 an...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2010-05, Vol.105 (3), p.353-355
Main Authors: Vital-Brazil, Juliana Magalhães, Balassiano, Ilana Teruszkin, de Oliveira, Fabiano Sutter, Costa, Alberto Dias de Souza, Hillen, Leandro, Pereira, Martha Maria
Format: Article
Language:English
Subjects:
Brazil
DNA, Bacterial - analysis
Leptospira
Leptospira - genetics
Leptospira - isolation & purification
Leptospira spp
Leptospira spp, multiplex PCR, water sample
multiplex PCR
PARASITOLOGY
Polymerase Chain Reaction - methods
Poverty Areas
TROPICAL MEDICINE
Water Microbiology
water sample
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Summary:The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.
ISSN:1678-8060
0074-0276
1678-8060
0074-0276
DOI:10.1590/s0074-02762010000300020