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Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 an...
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| Published in: | Memórias do Instituto Oswaldo Cruz 2010-05, Vol.105 (3), p.353-355 |
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| Main Authors: | , , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-b603t-a57b17b9a75bad02f8cab49bce9419e97205845fda6945f5f09d906dbdbf954b3 |
| container_end_page | 355 |
| container_issue | 3 |
| container_start_page | 353 |
| container_title | Memórias do Instituto Oswaldo Cruz |
| container_volume | 105 |
| creator | Vital-Brazil, Juliana Magalhães Balassiano, Ilana Teruszkin de Oliveira, Fabiano Sutter Costa, Alberto Dias de Souza Hillen, Leandro Pereira, Martha Maria |
| description | The aim of this study was to apply a molecular protocol to detect
leptospiral DNA in environmental water samples. The study was carried
out in a peri-urban settlement in Petrópolis, state of Rio de
Janeiro. A multiplex PCR method employing the primers LipL32 and
16SrRNA was used. Three out of 100 analysed samples were positive in
the multiplex PCR, two were considered to have saprophytic leptospires
and one had pathogenic leptospires. The results obtained supported the
idea that multiplex PCR can be used to detect Leptospira spp in water
samples. This method was also able to differentiate between saprophytic
and pathogenic leptospires and was able to do so much more easily than
conventional methodologies. |
| doi_str_mv | 10.1590/s0074-02762010000300020 |
| format | article |
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leptospiral DNA in environmental water samples. The study was carried
out in a peri-urban settlement in Petrópolis, state of Rio de
Janeiro. A multiplex PCR method employing the primers LipL32 and
16SrRNA was used. Three out of 100 analysed samples were positive in
the multiplex PCR, two were considered to have saprophytic leptospires
and one had pathogenic leptospires. The results obtained supported the
idea that multiplex PCR can be used to detect Leptospira spp in water
samples. This method was also able to differentiate between saprophytic
and pathogenic leptospires and was able to do so much more easily than
conventional methodologies.</description><identifier>ISSN: 1678-8060</identifier><identifier>ISSN: 0074-0276</identifier><identifier>EISSN: 1678-8060</identifier><identifier>EISSN: 0074-0276</identifier><identifier>DOI: 10.1590/s0074-02762010000300020</identifier><identifier>PMID: 20512254</identifier><language>eng</language><publisher>Brazil: Fundação Oswaldo Cruz, Fiocruz</publisher><subject>Brazil ; DNA, Bacterial - analysis ; Leptospira ; Leptospira - genetics ; Leptospira - isolation & purification ; Leptospira spp ; Leptospira spp, multiplex PCR, water sample ; multiplex PCR ; PARASITOLOGY ; Polymerase Chain Reaction - methods ; Poverty Areas ; TROPICAL MEDICINE ; Water Microbiology ; water sample</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2010-05, Vol.105 (3), p.353-355</ispartof><rights>Copyright © 2010 - Instituto Oswaldo Cruz - Fiocruz</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b603t-a57b17b9a75bad02f8cab49bce9419e97205845fda6945f5f09d906dbdbf954b3</citedby><cites>FETCH-LOGICAL-b603t-a57b17b9a75bad02f8cab49bce9419e97205845fda6945f5f09d906dbdbf954b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,24150,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20512254$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vital-Brazil, Juliana Magalhães</creatorcontrib><creatorcontrib>Balassiano, Ilana Teruszkin</creatorcontrib><creatorcontrib>de Oliveira, Fabiano Sutter</creatorcontrib><creatorcontrib>Costa, Alberto Dias de Souza</creatorcontrib><creatorcontrib>Hillen, Leandro</creatorcontrib><creatorcontrib>Pereira, Martha Maria</creatorcontrib><title>Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement</title><title>Memórias do Instituto Oswaldo Cruz</title><addtitle>Mem Inst Oswaldo Cruz</addtitle><description>The aim of this study was to apply a molecular protocol to detect
leptospiral DNA in environmental water samples. The study was carried
out in a peri-urban settlement in Petrópolis, state of Rio de
Janeiro. A multiplex PCR method employing the primers LipL32 and
16SrRNA was used. Three out of 100 analysed samples were positive in
the multiplex PCR, two were considered to have saprophytic leptospires
and one had pathogenic leptospires. The results obtained supported the
idea that multiplex PCR can be used to detect Leptospira spp in water
samples. This method was also able to differentiate between saprophytic
and pathogenic leptospires and was able to do so much more easily than
conventional methodologies.</description><subject>Brazil</subject><subject>DNA, Bacterial - analysis</subject><subject>Leptospira</subject><subject>Leptospira - genetics</subject><subject>Leptospira - isolation & purification</subject><subject>Leptospira spp</subject><subject>Leptospira spp, multiplex PCR, water sample</subject><subject>multiplex PCR</subject><subject>PARASITOLOGY</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Poverty Areas</subject><subject>TROPICAL MEDICINE</subject><subject>Water Microbiology</subject><subject>water sample</subject><issn>1678-8060</issn><issn>0074-0276</issn><issn>1678-8060</issn><issn>0074-0276</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqFUl2PFCEQnBiNd57-BeXNpzmbgYHh0WzUu2SNxo9nAkyPYcMMKzB-_Ptj3bvVxEQJpEmnqqhO0TTPKFzSXsGLDCB5C50UHVCoi9XTwb3mnAo5tAMIuP_H_ax5lPOuIiQT_GFz1kFPu67n583u7RqK3wf8Qd5vPrTWZBzJiAVd8XEhcSJb3JeY9z4Z4heCyzef4jLjUkwg303BRLKZq0Am0Rbjl8qfUpyJITmsM8lYSsAD_nHzYDIh45PbetF8fv3q0-aq3b57c715uW2tAFZa00tLpVVG9taM0E2DM5Yr61BxqlDJan7g_TQaoWrpJ1CjAjHa0U6q55ZdNNdH3TGand4nP5v0U0fj9a9GTF-0ScW7gFoKIcEKi45P3Co2KNaDYZYNE5NgRNW6PGpl5zFEvYtrWqp5_fEQgP4rgEp4fiTsU_y6Yi569tlhCGbBuGYteS9Zx6D7P5IxSpkCXpHyiHQp5pxwOg1FQR--wz_cPL19Y7UzjifeXf6_57M-hprdCeGSN_quGV3dVVdQdgMauL6z</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Vital-Brazil, Juliana Magalhães</creator><creator>Balassiano, Ilana Teruszkin</creator><creator>de Oliveira, Fabiano Sutter</creator><creator>Costa, Alberto Dias de Souza</creator><creator>Hillen, Leandro</creator><creator>Pereira, Martha Maria</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><general>Instituto Oswaldo Cruz, Ministério da Saúde</general><general>Fundação Oswaldo Cruz (FIOCRUZ)</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20100501</creationdate><title>Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement</title><author>Vital-Brazil, Juliana Magalhães ; 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leptospiral DNA in environmental water samples. The study was carried
out in a peri-urban settlement in Petrópolis, state of Rio de
Janeiro. A multiplex PCR method employing the primers LipL32 and
16SrRNA was used. Three out of 100 analysed samples were positive in
the multiplex PCR, two were considered to have saprophytic leptospires
and one had pathogenic leptospires. The results obtained supported the
idea that multiplex PCR can be used to detect Leptospira spp in water
samples. This method was also able to differentiate between saprophytic
and pathogenic leptospires and was able to do so much more easily than
conventional methodologies.</abstract><cop>Brazil</cop><pub>Fundação Oswaldo Cruz, Fiocruz</pub><pmid>20512254</pmid><doi>10.1590/s0074-02762010000300020</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1678-8060 |
| ispartof | Memórias do Instituto Oswaldo Cruz, 2010-05, Vol.105 (3), p.353-355 |
| issn | 1678-8060 0074-0276 1678-8060 0074-0276 |
| language | eng |
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| source | SciELO Brazil |
| subjects | Brazil DNA, Bacterial - analysis Leptospira Leptospira - genetics Leptospira - isolation & purification Leptospira spp Leptospira spp, multiplex PCR, water sample multiplex PCR PARASITOLOGY Polymerase Chain Reaction - methods Poverty Areas TROPICAL MEDICINE Water Microbiology water sample |
| title | Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement |
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