Analytical Separation of Closantel Enantiomers by HPLC

Closantel is an antiparasitic drug marketed in a racemic form with one chiral center. It is meaningful to develop a method for separating and analyzing the closantel enantiomers. In this work, two enantiomeric separation methods of closantel were explored by normal-phase high-performance liquid chro...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-11, Vol.26 (23), p.7288
Main Authors: Saleh, Basma, Ding, Tongyan, Wang, Yuwei, Zheng, Xiantong, Liu, Rong, He, Limin
Format: Article
Language:English
Subjects:
Acids - chemistry
Alcohols
Alcohols - chemistry
Amylose
Amylose - analogs & derivatives
Amylose - chemistry
Antiparasitic agents
Biological research
Carbamates (tradename)
chiral stationary phase
Chromatography
Chromatography, High Pressure Liquid
closantel
Drugs
enantiomeric separation
Enantiomers
Hexanes
High performance liquid chromatography
Influence
Ions
Isopropanol
Liquid chromatography
modifiers
Optimization
Pharmaceutical industry
Phase composition
Phenylcarbamates - chemistry
Retention
Salicylanilides - chemistry
Salicylanilides - isolation & purification
Separation
Stationary phase
Stereoisomerism
Temperature
Trifluoroacetic acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Closantel is an antiparasitic drug marketed in a racemic form with one chiral center. It is meaningful to develop a method for separating and analyzing the closantel enantiomers. In this work, two enantiomeric separation methods of closantel were explored by normal-phase high-performance liquid chromatography. The influences of the chiral stationary phase (CSP) structure, the mobile phase composition, the nature and proportion of different mobile phase modifiers (alcohols and acids), and the column temperature on the enantiomeric separation of closantel were investigated in detail. The two enantiomers were successfully separated on the novel CSP of isopropyl derivatives of cyclofructan 6 and -hexane-isopropanol-trifluoroacetic acid (97:3:0.1, / / ) as a mobile phase with a resolution ( s) of about 2.48. The enantiomers were also well separated on the CSP of -carbamates of amylose with a higher s (about 3.79) when a mixture of -hexane-isopropanol-trifluoroacetic acid (55:45:0.1, / / ) was used as mobile phase. Thus, the proposed separation methods can facilitate molecular pharmacological and biological research on closantel and its enantiomers.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26237288