Removal of aqueous phenol and phenol derivatives by immobilized potato polyphenol oxidase

Phenols containing halogens, which tend to deactivate the aromatic nuclei, constitute a significant category of highly toxic and difficult-to-degrade pollutants, which arise from a wide variety of industries. The main purpose of this study was to obtain an inexpensive immobilized enzyme for the remo...

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Bibliographic Details
Published in:Journal of the Serbian Chemical Society 2011-01, Vol.76 (4), p.513-522
Main Authors: Loncar, N, Bozic, N, Andelkovic, I, Milovanovic, A, Dojnov, B, Vujcic, M, Roglic, G, Vujcic, Z
Format: Article
Language:English
Subjects:
Categories
Derivatives
Eupergit
Halogens
immobilization
Phenol
Pollutants
Polyphenol oxidase
Polyphenylene oxides
potato
Potatoes
Solanum tuberosum
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Summary:Phenols containing halogens, which tend to deactivate the aromatic nuclei, constitute a significant category of highly toxic and difficult-to-degrade pollutants, which arise from a wide variety of industries. The main purpose of this study was to obtain an inexpensive immobilized enzyme for the removal of phenols. Partially purified potato polyphenol oxidase (PPO) was immobilized onto different commercial and laboratory produced carriers. Three of the obtained biocatalysts, with the highest PPO activities, namely Eupergit C250L-PPO; Celite-PPO and CelluloseM-PPO, were tested in a batch reactor for the removal of phenol, 4-chlorophenol and 4-bromophenol. In the case of 2.5 mM substrates with Eupergit C250L-PPO, an around 45% removal of 4-bromophenol was achieved, while the removals 4-chlorophenol and phenol were 35 and 20%, respectively. The reusability of Eupergit C250L-PPO for the removal of 4-chlorophenol was tested. After eight repeated tests, the efficiency of 4-chlorophenol removal by Eupergit C250L-PPO immobilisate had decreased to 55%.
ISSN:0352-5139
1820-7421
DOI:10.2298/JSC100619046L