Optineurin deficiency in mice contributes to impaired cytokine secretion and neutrophil recruitment in bacteria-driven colitis

• Published in: Disease models & mechanisms 2015-08, Vol.8 (8), p.817-829
• Main Authors: Chew, Thean S, O'Shea, Nuala R, Sewell, Gavin W, Oehlers, Stefan H, Mulvey, Claire M, Crosier, Philip S, Godovac-Zimmermann, Jasminka, Bloom, Stuart L, Smith, Andrew M, Segal, Anthony W
• Format: Article
• Language: English
• Subjects: Adult; Amyotrophic lateral sclerosis; Animals; Autophagy; Bacteria; Bacteria - metabolism; Bacterial infections; Case-Control Studies; Cell Cycle Proteins; Citrobacter; Citrobacter - physiology; Colitis - blood; Colitis - microbiology; Colitis - pathology; Crohn Disease - genetics; Crohn Disease - microbiology; Crohn's disease; Cytokines; Cytokines - blood; Cytokines - metabolism; Danio rerio; E coli; Escherichia coli; Escherichia coli - physiology; Escherichia coli Infections - prevention & control; Eye Proteins - metabolism; Female; Gene expression; Golgi Apparatus - metabolism; Humans; Inflammation; Inflammation Mediators - metabolism; Inflammatory bowel disease; Inflammatory diseases; Inheritance Patterns - genetics; Macrophages; Macrophages - metabolism; Male; Membrane Transport Proteins; Mice; Middle Aged; Models, Biological; Mutation; Neutrophil Infiltration; Neutrophils; Pagets disease; Patients; Polymorphism, Single Nucleotide - genetics; Recruitment; Salmonella; Small intestine; TNFα; Transcription Factor TFIIIA - deficiency; Transcription Factor TFIIIA - metabolism; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Up-Regulation; Zebrafish
• ISSN: 1754-8403; 1754-8411; 1754-8411; 1754-8403
• DOI: 10.1242/dmm.020362

Crohn's disease (CD) is associated with delayed neutrophil recruitment and bacterial clearance at sites of acute inflammation as a result of impaired secretion of proinflammatory cytokines by macrophages. To investigate the impaired cytokine secretion and confirm our previous findings, we performed transcriptomic analysis in macrophages and identified a subgroup of individuals with CD who had low expression of the autophagy receptor optineurin (OPTN). We then clarified the role of OPTN deficiency in: macrophage cytokine secretion; mouse models of bacteria-driven colitis and peritonitis; and zebrafish Salmonella infection. OPTN-deficient bone-marrow-derived macrophages (BMDMs) stimulated with heat-killed Escherichia coli secreted less proinflammatory TNFα and IL6 cytokines despite similar gene transcription, which normalised with lysosomal and autophagy inhibitors, suggesting that TNFα is mis-trafficked to lysosomes via bafilomycin-A-dependent pathways in the absence of OPTN. OPTN-deficient mice were more susceptible to Citrobacter colitis and E. coli peritonitis, and showed reduced levels of proinflammatory TNFα in serum, diminished neutrophil recruitment to sites of acute inflammation and greater mortality, compared with wild-type mice. Optn-knockdown zebrafish infected with Salmonella also had higher mortality. OPTN plays a role in acute inflammation and neutrophil recruitment, potentially via defective macrophage proinflammatory cytokine secretion, which suggests that diminished OPTN expression in humans might increase the risk of developing CD.