Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea. A model of sickle cell complication

Abstract Purpose: To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea. Methods: Eighteen male albino Wistar rats were divided into three...

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Bibliographic Details
Published in:Acta cirúrgica brasileira 2020, Vol.35 (3)
Main Authors: Santos Neto, Abilio Torres dos, Silva, Iandara Schettert, Ivo, Maria Lucia, Rodrigues, Camila Tozaki, Parisotto, Eduardo Benedetti, Ramalho, Rondon Tosta, Monteiro, Geanlucas Mendes
Format: Article
Language:English
Subjects:
Anemia
Hydroxyurea
NG-Nitroarginine Methyl Ester
Oxidative Stress
Rats
Sickle Cell
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Summary:Abstract Purpose: To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea. Methods: Eighteen male albino Wistar rats were divided into three groups. NG-nitro-L-arginine methyl ester (L-NAME) was intraperitoneally administered to induce oxidative stress. TBARS and plasma nitric oxide levels were analyzed in all groups. Histopathology of the liver and vascular tissue was performed. Results: Statistically significant differences were seen in liver, brain and spinal cord TBARS levels. Conclusions: Following the use of L-NAME, hepatic tissue increased the number of Kupffer cells as oxidative stress and inflammatory response increased. The use of L-NAME caused an increase in lipid peroxidation products and, consequently, in oxidative stress in animals. Hydroxyurea doses of 35 mg / kg / day reduced TBARS values in liver, brain and spinal cord.
ISSN:0102-8650
1678-2674
DOI:10.1590/s0102-865020200030000001