TGFß Gene Members and Their Regulatory Factors in Granulosa Compared to Cumulus Cells in PCOS: A Case-Control Study

Objective: Transforming growth factor-beta (TGF-β) superfamily and its members that include bone morphogenetic protein 15 (BMP15), anti-Mullerian hormone (AMH), growth /differentiation factor-9 (GDF9), and their respective receptors: BMPR1A, BMPR1B, and BMPR2 have been implicated as key regulators i...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2022-07, Vol.24 (7), p.410-416
Main Authors: Alvandian, Faezeh, Hosseini, Elham, Hashemian, Zohre, Khosravifar, Mona, Movaghar, Bahar, Shahhosein, Maryam, Shiva, Marzieh, Afsharian, Parvaneh
Format: Article
Language:English
Subjects:
Bone morphogenetic protein receptor type II
cumulus cell
Gametocytes
Gene expression
Genes
granulosa cell
Growth factors
In vitro fertilization
Original
Ovaries
polycystic ovarian syndrome
Polycystic ovary syndrome
Polymerase chain reaction
Receptors
Reproductive status
Stimulation
tgf-beta superfamily
Transforming growth factor-b
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Summary:Objective: Transforming growth factor-beta (TGF-β) superfamily and its members that include bone morphogenetic protein 15 (BMP15), anti-Mullerian hormone (AMH), growth /differentiation factor-9 (GDF9), and their respective receptors: BMPR1A, BMPR1B, and BMPR2 have been implicated as key regulators in various aspects of ovarian function. The abnormal function of the ovaries is one of the main contributing factors to polycystic ovarian syndrome (PCOS), so this study aimed to investigate the mRNA expression profile of these factors in granulosa (GCs) and cumulus cells (CCs) of those patients. Materials and Methods:The case-control research was conducted on 30 women (15 infertile PCOS and 15 normo-ovulatory patients, 22≤age ≤38 years old) who underwent ovarian stimulation for in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI) cycle. GCs/CCs were obtained during ovarian puncture. The expression analysis of the aforementioned genes was quantified using real-time polymerase chain reaction (PCR). Results: AMH and BMPR1A expression levels were significantly increased in GCs of PCOS compared to the control group. In contrast, GDF9, BMP15, BMPR1B, and BMPR2 expressions were decreased. PCOS' CC showed the same expression patterns. GDF9 and AMH were effectively expressed in normal CCs, and BMP15 and BMPR1B in normal GCs (P
ISSN:2228-5806
2228-5814
DOI:10.22074/cellj.2022.8051