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SPR Analysis of SUMO-Murine Rap1-Interacting Factor 1 C-Terminal Domain Interaction with G4

One of the advantages of surface plasmon resonance is its sensitivity and real-time analyses performed by this method. These characteristics allow us to further investigate the interactions of challenging proteins like Rap1-interacting factor 1 (Rif1). Rif1 is a crucial protein responsible for regul...

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Published in:Biosensors (Basel) 2022-01, Vol.12 (1), p.37
Main Authors: Alavi, Sana, Ghadiri, Hamed, Dabirmanesh, Bahareh, Khajeh, Khosro
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description One of the advantages of surface plasmon resonance is its sensitivity and real-time analyses performed by this method. These characteristics allow us to further investigate the interactions of challenging proteins like Rap1-interacting factor 1 (Rif1). Rif1 is a crucial protein responsible for regulating different cellular processes including DNA replication, repair, and transcription. Mammalian Rif1 is yet to be fully characterized, partly because it is predicted to be intrinsically disordered for a large portion of its polypeptide. This protein has recently been the target of research as a potential biomarker in many cancers. Therefore, finding its most potent interacting partner is of utmost importance. Previous studies showed Rif1's affinity towards structured DNAs and amongst them, T G was superior. Recent studies have shown mouse Rif1 (muRif1) C-terminal domain's (CTD) role in binding to G-quadruplexes (G4). There were many concerns in investigating the Rif1 and G4 interaction, which can be minimized using SPR. Therefore, for the first time, we have assessed its binding with G4 at nano-molar concentrations with SPR which seems to be crucial for its binding analyses. Our results indicate that muRif1-CTD has a high affinity for this G4 sequence as it shows a very low K (6 ± 1 nM).
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source Publicly Available Content Database; PubMed Central
subjects Affinity
Amino acids
Animals
Binding
Biomarkers
Biosensors
Cell cycle
dissociation constant (KD)
DNA biosynthesis
DNA repair
DNA Replication - physiology
Domains
G-quadruplex
G-Quadruplexes
Heat
Investigations
Mammals
Medical prognosis
Mice
molecular interaction
mouse Rap1-interacting factor 1
Polypeptides
Protein Binding
Proteins
Rap1 protein
RNA polymerase
Spectrum analysis
Surface Plasmon Resonance
Telomere-Binding Proteins - genetics
Telomere-Binding Proteins - metabolism
Transcription
title SPR Analysis of SUMO-Murine Rap1-Interacting Factor 1 C-Terminal Domain Interaction with G4
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