3D chick embryo chorioallantoic membrane model as an in vivo model to study morphological and histopathological features of feline fibrosarcomas

The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform preclinical oncological model for observing pro- and antiangiogenic response, tumor biology and metastasis. The main objective of this article was to present the modificatio...

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Bibliographic Details
Published in:BMC veterinary research 2017-06, Vol.13 (1), p.201-201, Article 201
Main Authors: Zabielska-Koczywąs, Katarzyna, Wojtkowska, Agata, Dolka, Izabella, Małek, Anna, Walewska, Magdalena, Wojtalewicz, Anna, Żbikowski, Artur, Lechowski, Roman
Format: Article
Language:English
Subjects:
Animals
Antiangiogenics
Antigens
Biological assay
Bone cancer
Breast cancer
Cancer therapies
Cat Diseases - pathology
Cats
Cell Line, Tumor
Cell lines
Chemotherapy
Chick Embryo
Chorioallantoic membrane
Chorioallantoic Membrane - pathology
Diseases
Drug delivery systems
Eggs
Embryos
Experimental animal model
in ovo assay
Feline fibrosarcoma
Fibrosarcoma
Fibrosarcoma - pathology
Fibrosarcoma - veterinary
Histopathology
Humidity
Immunohistochemistry
Internet
Ki-67
Kinases
Malignancy
Medical prognosis
Medical research
Metastases
Methods
Morphology
Neoplasm Grading
PCNA
Performance evaluation
Physiological aspects
Proliferating cell nuclear antigen
Proteins
Studies
Survival analysis
Tumors
Veterinary medicine
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Description
Summary:The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform preclinical oncological model for observing pro- and antiangiogenic response, tumor biology and metastasis. The main objective of this article was to present the modification of the CAM assay in order to evaluate tumor growth from two feline fibrosarcoma cell lines (FFS1, FFS3) and describe their morphological and histopathological features. The authors described morphological and histopathological features of two feline fibrosarcoma cell lines (FFS1 and FFS3) grown on the CAM. Tumors from the FFS1 cell line showed high malignancy (grade III), while tumors from the FFS3 cell line were grade II. Proliferation markers (Ki-67 and PCNA) were determined and the positive correlation between PCNA and tumor grade (r = 0.8247; p 
ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-017-1114-4