Exopolysaccharide Characterization of Rhizobium favelukesii LPU83 and Its Role in the Symbiosis With Alfalfa

• Published in: Frontiers in plant science 2021-02, Vol.12, p.642576-642576
• Main Authors: Castellani, Lucas G, Luchetti, Abril, Nilsson, Juliet F, Pérez-Giménez, Julieta, Wegener, Caren, Schlüter, Andreas, Pühler, Alfred, Lagares, Antonio, Brom, Susana, Pistorio, Mariano, Niehaus, Karsten, Torres Tejerizo, Gonzalo A
• Format: Article
• Language: English
• Subjects: alfalfa; exopolysaccharide; nitrogen fixation; Plant Science; rhizobia; symbiosis
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2021.642576

One of the greatest inputs of available nitrogen into the biosphere occurs through the biological N -fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and , as bacteria that lack EPS are unable to infect the host plants. LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in , but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in , is split into three different sections in , which probably arose by a recent event of horizontal gene transfer. A strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis.