Metformin Attenuates Ischemia-reperfusion Injury of Fatty Liver in Rats Through Inhibition of the TLR4/NF-κB Axis

Donor organs for liver transplantation may often have fatty liver disease, which confers a higher sensitivity to ischemia/reperfusion injury. At present, there is no effective treatment for the condition. Evidence has suggested that metformin, the first-line medication for diabetes, has protective e...

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Bibliographic Details
Published in:Balkan medical journal 2020-06, Vol.37 (4), p.196-202
Main Authors: Li, Xiaohua, Wang, Liangliang, Yang, Xiaoguang, Huang, Chunyan
Format: Article
Language:English
Subjects:
Animals
fatty liver
Fatty Liver - drug therapy
Fatty Liver - prevention & control
Hypoglycemic Agents - therapeutic use
inflammation
ischemia-reperfusion injury
liver transplantation
metformin
Metformin - therapeutic use
Original
Protective Factors
Rats
Rats, Sprague-Dawley
Reperfusion Injury - drug therapy
Reperfusion Injury - prevention & control
Tıp
Toll-Like Receptor 4 - drug effects
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Summary:Donor organs for liver transplantation may often have fatty liver disease, which confers a higher sensitivity to ischemia/reperfusion injury. At present, there is no effective treatment for the condition. Evidence has suggested that metformin, the first-line medication for diabetes, has protective effects against many disorders. However, the potential role of metformin in ischemia/reperfusion injury in fatty liver disease remains unclear. To examine the effect of metformin treatment during ischemia/reperfusion injury in fatty liver and determine the possible mechanisms. Animal experimentation. Sprague-Dawley male rats were fed a high-fat diet (520 kcal/100 g) for 14 weeks and then were subjected to the orthotopic autologous liver transplantation model. Sections of liver tissue were stained with hematoxylin and eosin to visualize the damage. Blood and liver samples were used to analyze the related proteins and components involved in the inflammatory signaling pathway. We found that metformin significantly ameliorated the ischemia/reperfusion injury of the fatty liver through a reduction in alanine aminotransferase/aspartate aminotransferase concentrations in the serum and a decrease in dead cells, as shown by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (p
ISSN:2146-3123
2146-3131
2146-3123
DOI:10.4274/balkanmedj.galenos.2020.2019.9.31