Effect of NK4 transduction in bone marrow-derived mesenchymal stem cells on biological characteristics of pancreatic cancer cells

Pancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a veh...

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Bibliographic Details
Published in:International journal of molecular sciences 2014-03, Vol.15 (3), p.3729-3745
Main Authors: Sun, Yun-Peng, Zhang, Ben-Long, Duan, Jian-Wen, Wu, Huan-Huan, Wang, Ben-Quan, Yu, Zheng-Ping, Yang, Wen-Jun, Shan, Yun-Feng, Zhou, Meng-Tao, Zhang, Qi-Yu
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Animals
Apoptosis
Blotting, Western
Bone marrow
bone marrow-derived mesenchymal stem cells
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cells, Cultured
Cellular biology
Coculture Techniques
Gene Expression
Genetic Vectors - genetics
HEK293 Cells
Hepatocyte Growth Factor - genetics
Hepatocyte Growth Factor - metabolism
Humans
Mesenchymal Stem Cells - metabolism
NK4
Pancreatic cancer
Pancreatic Neoplasms - pathology
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Signal transduction
Stem cells
transduction
Transduction, Genetic
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Summary:Pancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a vehicle of gene therapy for targeting pancreatic cancer. First, we successfully extracted MSCs from SD rats. Next, MSCs were efficiently transduced with NK4, an antagonist of hepatocyte growth factor (HGF) which comprising the N-terminal and the subsequent four kringle domains of HGF, by an adenoviral vector. Then, we confirmed that rat MSCs preferentially migrate to pancreatic cancer cells. Last, MSCs expressing NK4 (NK4-MSCs) strongly inhibited proliferation and migration of the pancreatic cancer cell line SW1990 after co-culture. These results indicate that MSCs can serve as a vehicle of gene therapy for targeting pancreatic cancer.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms15033729