Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume

To explore the transformation mechanisms of free gastrodin and combined gastrodin before and after steaming of ( ), a fresh sample was processed by the traditional steaming method prescribed by Chinese Pharmacopoeia (2015 version), and HPLC-ESI-TOF/MS method was used to identify the chemical composi...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-08, Vol.24 (17), p.3159
Main Authors: Li, Yun, Liu, Xiao-Qian, Liu, Shan-Shan, Liu, Da-Hui, Wang, Xiao, Wang, Zhi-Min
Format: Article
Language:English
Subjects:
Alcohol
Benzyl Alcohols - analysis
Chinese medicine
Chromatography, High Pressure Liquid - methods
Dose-response effects
Gastrodia - chemistry
Gastrodia elata
gastrodin
Glucosides - analysis
Glucosides - chemistry
Hydrolysis
Hypotheses
Ingredients
Mass spectrometry
Mass spectroscopy
Medical research
Organic chemistry
parishin A
Pharmacology
Quality assessment
Researchers
Spectrometry, Mass, Electrospray Ionization - methods
Steam
Steaming
Tandem Mass Spectrometry
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Summary:To explore the transformation mechanisms of free gastrodin and combined gastrodin before and after steaming of ( ), a fresh sample was processed by the traditional steaming method prescribed by Chinese Pharmacopoeia (2015 version), and HPLC-ESI-TOF/MS method was used to identify the chemical composition in steamed and fresh . Finally, 25 components were identified in based on the characteristic fragments of the compounds and the changes of the 25 components of fresh and steamed were compared by the relative content. Hydrolysis experiments and enzymatic hydrolysis experiments of 10 monomer compounds simulating the steaming process were carried out for the first time. As a result, hydrolysis experiments proved that free gastrodin or -hydroxybenzyl alcohol could be obtained by breaking ester bond or ether bond during the steaming process of . Enzymatic experiments showed that steaming played an important role in the protection of gastrodin, confirming the hypothesis that steaming can promote the conversion of chemical constituents of -inhibiting enzymatic degradation. This experiment clarified the scientific mechanism of the traditional steaming method of and provided reference for how to apply decoction to some extent.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24173159