The Use of Human Platelet Lysate as a Coating Substance for Adipose-Derived Stem Cell Expansion

Large-scale production of mesenchymal stromal cells is essential for sufficient therapeutic doses in regenerative medicine. However, long-term cultivation encounters limited cell growth and cellular aging. Therefore, an alternative cell culture approach that promotes proliferation and attenuates cel...

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Bibliographic Details
Published in:Frontiers in bioscience (Landmark. Print) 2024-02, Vol.29 (2), p.88-88
Main Authors: Tragoonlugkana, Patcharapa, Chitchongyingcharoen, Narong, Pruksapong, Chatchai, Hassan, Shirmeela, Ngamkham, Kamolchanok, Kamprom, Witchayapon, Supokawej, Aungkura
Format: Article
Language:English
Subjects:
adipose-derived stem cell
Blood Platelets - metabolism
Cell Culture Techniques
Cell Differentiation
Cell Proliferation
cell senescence
Cells, Cultured
coating substance
human platelet lysate
Humans
Mesenchymal Stem Cells - metabolism
mesenchymal stromal cell
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Summary:Large-scale production of mesenchymal stromal cells is essential for sufficient therapeutic doses in regenerative medicine. However, long-term cultivation encounters limited cell growth and cellular aging. Therefore, an alternative cell culture approach that promotes proliferation and attenuates cell senescence is required. Human platelet lysate (HPL) is a potent supplement for cell expansion. Applying HPL as a coating material can potentially improve mesenchymal stromal cell cultures. To examine the capacity of HPL, it was used to pre-coat a tissue culture plate for adipose-derived mesenchymal stromal cell expansion. Alterations in biological features of adipose-derived stem cells (ADSCs) were investigated, including cell adhesion assays, cell proliferation, population doubling time, and cellular senescence. ADSCs cultured on HPL-coated plates significantly increased cell adhesion rate, shortened population doubling time, and stimulated cell growth. The senescent cells were significantly decreased in ADSCs cultured in an HPL-coated plate, and the expression levels of senescence-associated genes, including , , and , were downregulated. Furthermore, Western blotting analysis revealed that HPL was enriched with fibronectin and vitronectin, essential cell adhesive proteins. HPL was effectively used as a coating material for ADSC expansions. Cellular cultivation on the HPL coating is an alternative approach for producing mesenchymal stromal cells.
ISSN:2768-6701
2768-6698
DOI:10.31083/j.fbl2902088