Development of a functional dairy snack containing oleoylethanolamide that reduces food intake in normal-weight and obese minipigs

• Published in: Journal of functional foods 2023-12, Vol.111, p.105916, Article 105916
• Main Authors: Boudry, Gaëlle, Mennella, Ilario, Menard, Olivia, Janvier, Régis, Nogret, Isabelle, Madadlou, Ashkan, Cahu, Armelle, Le Normand, Laurence, Bobillier-Chaumont, Eric, Ferracane, Rosalia, Vitaglione, Paola, Dupont, Didier, Val-Laillet, David
• Format: Article
• Language: English
• Subjects: Food and Nutrition; Food structure; In vitro digestion; Life Sciences; N-acylethanolamine; Regulation of food intake
• ISSN: 1756-4646; 1756-4646
• DOI: 10.1016/j.jff.2023.105916

Oleoylethanolamide (OEA) is a safe bioactive lipid that demonstrated strong anorexigenic properties in preclinical and clinical models. In order to evaluate the importance of OEA delivery kinetic on its anorectic properties, we developed OEA-containing dairy snacks with either a liquid or a semi-solid form. The OEA+ liquid snack, but not the semi-solid one, reduced by 14 and 18 % the amount of feed eaten by normal-weight and obese minipigs, respectively, in an eating behavior test performed 4 h after snack ingestion. In vitro digestion experiments revealed that OEA release in intestinal digesta was greatly enhanced when the snack was liquid compared to the semi-solid structure. Kinetic investigations of several plasma parameters after liquid snack ingestion points towards different potential mechanism depending upon the minipig weight status, with an effect of the OEA+ liquid snack likely on endocannabinoid and other related N-acylethanolamine metabolism in normal-weight minipigs and on ketogenesis in obese ones.