Isolation and purification of oridonin from the whole plant of Isodon rubescens by high-speed counter-current chromatography

Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, coll...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2011-09, Vol.16 (9), p.7949-7957
Main Authors: He, Fa, Bai, Yuhua, Wang, Jing, Wei, Jing, Yu, Chunyue, Li, Sen, Yang, Weili, Han, Chenghua
Format: Article
Language:English
Subjects:
Cancer
Centrifuges
Chromatography
Chromatography, High Pressure Liquid - methods
Diterpenes, Kaurane - analysis
Diterpenes, Kaurane - chemistry
Diterpenes, Kaurane - isolation & purification
Esophagus
high-speed counter-current chromatography (HSCCC)
Isodon - chemistry
Isodon rubescens
Magnetic Resonance Spectroscopy
Natural products
Optimization
oridonin
Plant Extracts - analysis
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Solvents
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Summary:Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, 1H-NMR and 13C-NMR.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules16097949