Nanomechanical resilience and thermal stability of RSJ2 phage

• Published in: Scientific reports 2024-08, Vol.14 (1), p.19389-10, Article 19389
• Main Authors: Sae-Ueng, Udom, Bunsuwansakul, Chooseel, Showpanish, Kittiya, Phironrit, Namthip, Thadajarassiri, Jidapa, Nehls, Christians
• Format: Article
• Language: English
• Subjects: 631/57; 639/766; 639/925; Adaptation; Atomic force microscope; Atomic force microscopy; Bacteria; Bacteriophage; Bacteriophages - physiology; Biological control; Crop diseases; E coli; Environmental factors; Genetically altered foods; Green economy; Humanities and Social Sciences; Infections; Infectivity; Lysis; Mechanical properties; Microscopy; Microscopy, Atomic Force; Morphology; multidisciplinary; Phage infectivity; Phage stiffness; Phages; Protein structure; Ralstonia solanacearum - virology; RSJ2 phage; Science; Science (multidisciplinary); Sustainable agriculture; Systematic review; Temperature; Temperature effects; Temperature requirements; Thailand; Thermal stability; Tropical environment; Tropical environments; Viruses; Wilt
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-70056-8

As the world moves toward a green economy and sustainable agriculture, bacterial viruses or bacteriophages (phages) become attractive biocontrol agents for controlling crop diseases. Effective utilization of phages in farms requires integrated knowledge of crops, pathogens, phages, and surroundings. Phages must encounter environmental fluctuations, including temperature, and must remain infectious for successful bacteria lysis. This work studied a soilborne RSJ2 phage discovered in Thailand, which can eliminate Ralstonia solanacearum , causing bacterial wilt disease in chili. We investigated how phage infectivity and nanomechanics responded to thermal changes. The plaque-based assay showed that the infectivity of the RSJ2 phage was stable within 24–40 °C, an average temperature fluctuation in tropical regions. The structural examination also showed that the phage remained intact. The nanomechanical property of the phage was inspected by the atomic force microscopy-based nanoindentation. The result revealed that the phage stiffness within 24–40 °C was statistically similar (0.05–0.06 N/m). Upon heating at 40 °C for 1, 5, and 10 h and resting at 25 °C, the stiffness of the phage particles increased to 0.09–0.11 N/m (54–83% increase). The stiffness results suggest structural adaptation of the protein subunits as a response to thermal alteration. The study exhibits that the phage structure is highly dynamic and can nanomechanically respond to varying temperatures. The phage stiffness may reveal insight into phage adaptation to environmental factors. Equipped with the knowledge of phage infectivity, structure, and nanomechanics, we can design practical guidelines for effective phage usage in farming and propelling green and safe agriculture.