A biological extract of turmeric (Curcuma longa) modulates response of cartilage explants to lipopolysaccharide

Turmeric is commonly used as a dietary treatment for inflammation, but few studies have evaluated the direct effect of turmeric on cartilage. The purpose of this study was to characterize cartilage explants' inflammatory responses to lipopolysaccharide in the presence of a simulated biological...

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Bibliographic Details
Published in:BMC complementary and alternative medicine 2019-09, Vol.19 (1), p.252-252, Article 252
Main Authors: Pearson, Wendy, Kott, Laima S
Format: Article
Language:English
Subjects:
Alternative medicine
Animals
Anti-Inflammatory Agents - pharmacology
Arthritis
Calcein
Care and treatment
Cartilage
Cartilage - drug effects
Cartilage - immunology
Cartilage inflammation
Cell culture
Costochondritis
Culture media
Curcuma - chemistry
Diet
Dinoprostone - immunology
Explants
Fluorescence
Glycosaminoglycans
Glycosaminoglycans - immunology
Hepatic metabolism
In Vitro Techniques
Inflammation
Intestine
Lipopolysaccharides
Lipopolysaccharides - adverse effects
Lipopolysaccharides - immunology
Male
Methylene blue
Microsomes
Mitogens
Mucopolysaccharides
NADP
Nitric oxide
Nitric Oxide - immunology
Nitrogen oxides
Plant extracts
Plant Extracts - pharmacology
Prostaglandin E2
Prostaglandins
Rats
Simulated digestion
Swine
Tissue culture
Turmeric
Variance analysis
Viability
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Summary:Turmeric is commonly used as a dietary treatment for inflammation, but few studies have evaluated the direct effect of turmeric on cartilage. The purpose of this study was to characterize cartilage explants' inflammatory responses to lipopolysaccharide in the presence of a simulated biological extract of turmeric. Turmeric was incubated in simulated gastric and intestinal fluid, followed by inclusion of liver microsomes and NADPH. The resulting extract (TUR ) was used to condition cartilage explants in the presence or absence of lipopolysaccharide. Explants were cultured for 96 h (h); the first 24 h in basal tissue culture media and the remaining 72 h in basal tissue culture media containing TUR (0, 3, 9 or 15 μg/mL). Lipopolysaccharide (0 or 5 μg/mL) was added for the final 48 H. media samples were collected immediately prior to lipopolysaccharide exposure (0 h) and then at 24 and 48 h after, and analyzed for prostaglandin E (PGE ), glycosaminoglycan (GAG), and nitric oxide (NO). Explants were stained with calcein-AM for an estimate of live cells. Data were analyzed using a 2-way repeated measures (GAG, PGE , NO) or 1-way ANOVA without repeated measures (viability). Significance accepted at p 
ISSN:1472-6882
1472-6882
DOI:10.1186/s12906-019-2660-z