Immune reactivity of Brucella melitensis-vaccinated rabbit serum with recombinant Omp31 and DnaK proteins

Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein (DnaK) and an outer membrane prote...

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Bibliographic Details
Published in:Iranian journal of microbiology 2013-03, Vol.5 (1), p.19-23
Main Authors: Ghasemi, Amir, Salari, Mohammad Hossein, Zarnani, Amir Hassan, Pourmand, Mohammad Reza, Ahmadi, Hojat, Mirshafiey, Abbas, Jeddi-Tehrani, Mahmood
Format: Article
Language:English
Subjects:
Brucella
Cloning
ELISA
Immune Reactivity
Medicin och hälsovetenskap
Original
Protein Expression
Purification
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Summary:Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein (DnaK) and an outer membrane protein (Omp31) of B. melitensis were cloned, expressed in E.coli BL21 and then purified using Ni-NTA agarose. Immunized serum was prepared from a rabbit inoculated with attenuated B. melitensis. It was proved that immunized serum contains antibodies against recombinant Omp31 (rOmp31) and DnaK (rDnaK) by Western blot and ELISA assays. The results may suggest the importance of these proteins as subunit vaccines against B. melitensis as well as targets for immunotherapy.
ISSN:2008-3289
2008-4447