Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes

The contribution of environmental pollutants to the obesity pandemic is still not yet fully recognized. Elucidating possible cellular and molecular mechanisms of their effects is of high importance. Our study aimed to evaluate the effect of chronic, 21-day-long, 2,2-bis (4-chlorophenyl)-1,1-dichlore...

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Published in:Biomolecules (Basel, Switzerland) Switzerland), 2021-07, Vol.11 (8), p.1068
Main Authors: Kladnicka, Iva, Cedikova, Miroslava, Jedlicka, Jan, Kohoutova, Michaela, Muller, Ludek, Plavinova, Iveta, Kripnerova, Michaela, Bludovska, Monika, Kuncova, Jitka, Mullerova, Dana
Format: Article
Language:English
Subjects:
Adipocytes
Adipogenesis
Cell differentiation
Diabetes
Electron transport
Energy
human adipose-derived mesenchymal stem cells
Lipids
Membrane potential
Mesenchymal stem cells
Metabolism
Metabolites
Mitochondria
mitochondrial respiration
Molecular modelling
Morphology
Obesity
Organochlorine compounds
Oxygen consumption
p,p′-DDE
Pandemics
Physiology
Pollutants
Respiration
Stem cells
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Summary:The contribution of environmental pollutants to the obesity pandemic is still not yet fully recognized. Elucidating possible cellular and molecular mechanisms of their effects is of high importance. Our study aimed to evaluate the effect of chronic, 21-day-long, 2,2-bis (4-chlorophenyl)-1,1-dichlorethylenedichlorodiphenyldichloroethylene (p,p′-DDE) exposure of human adipose-derived mesenchymal stem cells committed to adipogenesis on mitochondrial oxygen consumption on days 4, 10, and 21. In addition, the mitochondrial membrane potential (MMP), the quality of the mitochondrial network, and lipid accumulation in maturing cells were evaluated. Compared to control differentiating adipocytes, exposure to p,p′-DDE at 1 μM concentration significantly increased basal (routine) mitochondrial respiration, ATP-linked oxygen consumption and MMP of intact cells on day 21 of adipogenesis. In contrast, higher pollutant concentration seemed to slow down the gradual increase in ATP-linked oxygen consumption typical for normal adipogenesis. Organochlorine p,p′-DDE did not alter citrate synthase activity. In conclusion, in vitro 1 μM p,p′-DDE corresponding to human exposure is able to increase the mitochondrial respiration per individual mitochondrion at the end of adipocyte maturation. Our data reveal that long-lasting exposure to p,p′-DDE could interfere with the metabolic programming of mature adipocytes.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom11081068