Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora

• Published in: Molecules (Basel, Switzerland) Switzerland), 2017-08, Vol.22 (9), p.1449
• Main Authors: Reddy, Chinreddy Subramanyam, Lee, Sang-Hoon, Yoon, Jeong Su, Kim, Jae Kwang, Lee, Sang Won, Hur, Mok, Koo, Sung Cheol, Meilan, Jin, Lee, Woo Moon, Jang, Jae Ki, Hur, Yoonkang, Park, Sang Un, Kim, And Yeon Bok
• Format: Article
• Language: English
• Subjects: Accumulation; Asteraceae - genetics; Asteraceae - metabolism; Biosynthesis; Biosynthetic Pathways; Carotene; carotenoid; Carotenoids; Carotenoids - biosynthesis; Chemical compounds; Cloning; Cloning, Molecular; Desaturase; Gene Expression; Genes; Hepatitis; High performance liquid chromatography; Homology; Hydroxylase; Ixeris; Ixeris dentata var. albiflora; Leaves; Liquid chromatography; Lycopene; Organs; Pharmacology; Phytoene synthase; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant Roots - genetics; Plant Roots - metabolism; Plants (botany); transcript level; Transcription; Tumors; Zeaxanthin; Zeaxanthin epoxidase
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules22091449

var. is considered as a potential therapeutic agent against mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors as well as good seasoned vegetable in Far East countries. Phytoene synthase (PSY), phytoene desaturase (PDS) ξ-carotene desaturase (ZDS), lycopene β-cyclase (LCYB), lycopene ε-cyclase (LCYE), ε-ring carotene hydroxylase (CHXB), and zeaxanthin epoxidase (ZDS) are vital enzymes in the carotenoid biosynthesis pathway. We have examined these seven genes from a that are participated in carotenoid biosynthesis utilizing an Illumina/Solexa HiSeq 2000 platform. In silico analysis of the seven deduced amino acid sequences were revealed its closest homology with other Asteracea plants. Further, we explored transcript levels and carotenoid accumulation in various organs of using quantitative real time PCR and high-performance liquid chromatography, respectively. The highest transcript levels were noticed in the leaf for all the genes while minimal levels were noticed in the root. The maximal carotenoid accumulation was also detected in the leaf. We proposed that these genes expressions are associated with the accumulation of carotenoids. Our findings may suggest the fundamental clues to unravel the molecular insights of carotenoid biosynthesis in various organs of .