Cardiogel supports adhesion, proliferation and differentiation of stem cells with increased oxidative stress protection

Cultured murine bone marrow derived mesenchymal stem cells (BMSC) when grown along with cardiogel derived from mouse cardiac fibroblast, exhibited increased cell proliferation and differentiation and enhanced survival under oxidative stress induced by the exposure of H2O2 in vitro (similar to in viv...

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Bibliographic Details
Published in:European cells & materials 2011-01, Vol.21, p.107-121
Main Authors: Sreejit, P, Verma, R S
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
cardiogel
Cell Adhesion
Cell Differentiation
Cell Proliferation
Coronary Disease - therapy
differentiation
Extracellular Matrix
Fibroblasts
Materials Testing
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - physiology
Mice
Oxidative Stress
oxidative stress protection
proliferation
Stem Cell Transplantation
Stem cells
therapeutic biomaterial
Tissue Engineering
Tissue Scaffolds
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Summary:Cultured murine bone marrow derived mesenchymal stem cells (BMSC) when grown along with cardiogel derived from mouse cardiac fibroblast, exhibited increased cell proliferation and differentiation and enhanced survival under oxidative stress induced by the exposure of H2O2 in vitro (similar to in vivo ischemia like condition). Adhesion of BMSC to the cardiogel occurred at a faster rate when compared to the cells grown on normal surface. BMSC attached to cardiogel showed an increased resistance to proteolytic (enzymatic) disassociation. This is the first report on an attempt to use an in house biomaterial for the growth of BMSC that led to their heightened resistance towards oxidative stress. These studies support that cardiogel is an efficient biodegradable three-dimensional extracellular matrix which supports better growth of BMSC and can be used as a scaffold for stem cell delivery, with potential therapeutic applications in cardiac tissue regeneration.
ISSN:1473-2262
1473-2262
DOI:10.22203/eCM.v021a09