Protocol to engineer nanofilms embedded lipid nanoparticles for controlled and targeted drug delivery (NECTAR)

• Published in: STAR protocols 2023-03, Vol.4 (1), p.101685-101685, Article 101685
• Main Authors: Porwal, Rashi, Hayward, Stephen L., Kidambi, Srivatsan
• Format: Article
• Language: English
• Subjects: Biotechnology and bioengineering; Drug Delivery Systems - methods; Liposomes; Material sciences; Plant Nectar; Polyelectrolytes - chemistry; Protocol
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2022.101685

We present a protocol to engineer a substrate-mediated delivery platform comprising hyaluronic acid-coated lipid nanoparticles (HALNPs) embedded into polyelectrolyte multilayer (PEM) films. This platform allows controlled spatiotemporal release of lipid nanoparticles (LNP) by embedding them within the polyelectrolyte multilayer films matrix. HALNP conjugate with antibodies also adds the ability for targeted delivery. The use of LNP enables this platform to encapsulate both hydrophobic and hydrophilic drugs. This platform can easily be reproduced and utilized for various biomedical drug delivery applications. For complete details on the use and execution of this protocol, please refer to Hayward et al. (2015, 2016a, 2016b), Hayward and Kidambi (2018), and Kidambi and Hayward (2022). [Display omitted] •NECTAR platform for controlled spatiotemporal release of lipid nanoparticles (LNP)•Polyelectrolyte multilayer films embedded LNPs for controlling drug delivery•Hyaluronic acid-coated LNP (HALNP) conjugate with antibodies for targeted delivery•HALNP encapsulates both hydrophobic and hydrophilic drugs Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. We present a protocol to engineer a substrate-mediated delivery platform comprising hyaluronic acid-coated lipid nanoparticles (HALNPs) embedded into polyelectrolyte multilayer (PEM) films. This platform allows controlled spatiotemporal release of lipid nanoparticles (LNP) by embedding them within the polyelectrolyte multilayer films matrix. HALNP conjugate with antibodies also adds the ability for targeted delivery. The use of LNP enables this platform to encapsulate both hydrophobic and hydrophilic drugs. This platform can easily be reproduced and utilized for various biomedical drug delivery applications.