Piezoelectric Biosensor for a Simple Serological Diagnosis of Tularemia in Infected European Brown Hares (Lepus europaeus)

Piezoelectric biosensor was used for diagnosis of infection by Francisellatularensis subsp. holarctica in European brown hares. Two kinds of experiments wereperformed in this study. First, sera from experimentally infected European brown hares(Lepus europaeus) were assayed by piezoelectric biosensor...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2007-11, Vol.7 (11), p.2825-2834
Main Authors: Pohanka, Miroslav, Treml, František, Hubálek, Martin, Banďouchová, Hana, Beklová, Miroslava, Pikula, Jiří
Format: Article
Language:English
Subjects:
agglutination test
Biosensors
experimental and natural infection
Francisella tularensis
Full Research Paper
immunosensor
Infections
Pharmaceutical sciences
Proteins
reservoir host
Sensors
Serology
Taxonomy
Tularemia
Virulence
Zoonoses
zoonosis
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Summary:Piezoelectric biosensor was used for diagnosis of infection by Francisellatularensis subsp. holarctica in European brown hares. Two kinds of experiments wereperformed in this study. First, sera from experimentally infected European brown hares(Lepus europaeus) were assayed by piezoelectric biosensor and the seventh day postinfection was found as the first one when statistically significant diagnosis of tularemia waspossible; all other sera collected from hares later than on day 7 following the infection werefound tularemia positive. Typing to classify the field strain of F. tularensis used for theexperimental infection was confirmed by proteome study. Second, sera from 35 Europeanbrown hare specimens sampled at hunting grounds and tested as tularemia positive by slowagglutination allowed diagnosis of tularemia by the piezoelectric biosensor. All these sera ofnaturally infected hares were found as tularemia positive, too. Efficacy of the piezoelectricbiosensor for the serological diagnosis of tularemia is discussed.
ISSN:1424-8220
1424-8220
DOI:10.3390/s7112825