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Protective Effect of Lactobacillus crispatus against Vaginal Colonization with Group B Streptococci in the Third Trimester of Pregnancy
Background: A normal vaginal microbiota may protect the vaginal mucosa from colonization by potentially pathogenic bacteria, including group B streptococci (GBS). The aim of this study was to investigate the association between colonization with GBS and the presence of specific vaginal microbiota is...
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Published in: | Pathogens (Basel) 2022-08, Vol.11 (9), p.980 |
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description | Background: A normal vaginal microbiota may protect the vaginal mucosa from colonization by potentially pathogenic bacteria, including group B streptococci (GBS). The aim of this study was to investigate the association between colonization with GBS and the presence of specific vaginal microbiota isolated from vaginal swabs in the third trimester of pregnancy. Methods: A semiquantitative culture of 1860 vaginal swabs from consecutive pregnant women in their third trimester was analyzed. The dominant bacteria, including lactobacilli, were identified using MALDI-TOF mass spectrometry. An enrichment culture for GBS was performed on the swabs. GBS colonization correlated with the bacteria isolated at the same time. Results: Lactobacilluscrispatus was isolated in 27.5% of the cultures, followed by L. jensenii (13.9%), L. gasseri (12.6%), and L. iners (10.1%). The presence of lactobacilli as a group, and of L. crispatus, inversely correlated with GBS colonization (OR = 0.44 and OR = 0.5, respectively; both with p < 0.001). Other microorganisms, including Gardnerella vaginalis, mixed aerobic bacteria and yeasts, were not associated with GBS colonization. Conclusions: Lactobacilli, especially L. crispatus, may prevent GBS colonization in pregnancy. Maintaining a normal vaginal microbiota could be an effective method for the antibiotic-free prevention of invasive GBS infections in neonates. |
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The aim of this study was to investigate the association between colonization with GBS and the presence of specific vaginal microbiota isolated from vaginal swabs in the third trimester of pregnancy. Methods: A semiquantitative culture of 1860 vaginal swabs from consecutive pregnant women in their third trimester was analyzed. The dominant bacteria, including lactobacilli, were identified using MALDI-TOF mass spectrometry. An enrichment culture for GBS was performed on the swabs. GBS colonization correlated with the bacteria isolated at the same time. Results: Lactobacilluscrispatus was isolated in 27.5% of the cultures, followed by L. jensenii (13.9%), L. gasseri (12.6%), and L. iners (10.1%). The presence of lactobacilli as a group, and of L. crispatus, inversely correlated with GBS colonization (OR = 0.44 and OR = 0.5, respectively; both with p < 0.001). Other microorganisms, including Gardnerella vaginalis, mixed aerobic bacteria and yeasts, were not associated with GBS colonization. Conclusions: Lactobacilli, especially L. crispatus, may prevent GBS colonization in pregnancy. Maintaining a normal vaginal microbiota could be an effective method for the antibiotic-free prevention of invasive GBS infections in neonates.</description><identifier>ISSN: 2076-0817</identifier><identifier>EISSN: 2076-0817</identifier><identifier>DOI: 10.3390/pathogens11090980</identifier><identifier>PMID: 36145412</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Aerobic bacteria ; Aerobic microorganisms ; Antibiotics ; Bacteria ; Childbirth & labor ; Colonization ; Enrichment media ; Gestational age ; group B Streptococcus ; Lactobacilli ; Lactobacillus crispatus ; Mass spectrometry ; Mass spectroscopy ; Microbiota ; Microorganisms ; Neonates ; Pregnancy ; pregnant women ; Scientific imaging ; semiquantitative culture ; Streptococcus ; Streptococcus infections ; Vagina ; vaginal microbiota ; Womens health ; Yeasts</subject><ispartof>Pathogens (Basel), 2022-08, Vol.11 (9), p.980</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-b4a1e5bbe037a2b776d666a175770b4bfd7ad34abba1add230e11e1a4f0e4b583</citedby><cites>FETCH-LOGICAL-c493t-b4a1e5bbe037a2b776d666a175770b4bfd7ad34abba1add230e11e1a4f0e4b583</cites><orcidid>0000-0002-8373-8080</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2716581203/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2716581203?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36145412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Starc, Maja</creatorcontrib><creatorcontrib>Lučovnik, Miha</creatorcontrib><creatorcontrib>Eržen Vrlič, Petra</creatorcontrib><creatorcontrib>Jeverica, Samo</creatorcontrib><title>Protective Effect of Lactobacillus crispatus against Vaginal Colonization with Group B Streptococci in the Third Trimester of Pregnancy</title><title>Pathogens (Basel)</title><addtitle>Pathogens</addtitle><description>Background: A normal vaginal microbiota may protect the vaginal mucosa from colonization by potentially pathogenic bacteria, including group B streptococci (GBS). The aim of this study was to investigate the association between colonization with GBS and the presence of specific vaginal microbiota isolated from vaginal swabs in the third trimester of pregnancy. Methods: A semiquantitative culture of 1860 vaginal swabs from consecutive pregnant women in their third trimester was analyzed. The dominant bacteria, including lactobacilli, were identified using MALDI-TOF mass spectrometry. An enrichment culture for GBS was performed on the swabs. GBS colonization correlated with the bacteria isolated at the same time. Results: Lactobacilluscrispatus was isolated in 27.5% of the cultures, followed by L. jensenii (13.9%), L. gasseri (12.6%), and L. iners (10.1%). The presence of lactobacilli as a group, and of L. crispatus, inversely correlated with GBS colonization (OR = 0.44 and OR = 0.5, respectively; both with p < 0.001). Other microorganisms, including Gardnerella vaginalis, mixed aerobic bacteria and yeasts, were not associated with GBS colonization. Conclusions: Lactobacilli, especially L. crispatus, may prevent GBS colonization in pregnancy. Maintaining a normal vaginal microbiota could be an effective method for the antibiotic-free prevention of invasive GBS infections in neonates.</description><subject>Aerobic bacteria</subject><subject>Aerobic microorganisms</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Childbirth & labor</subject><subject>Colonization</subject><subject>Enrichment media</subject><subject>Gestational age</subject><subject>group B Streptococcus</subject><subject>Lactobacilli</subject><subject>Lactobacillus crispatus</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Neonates</subject><subject>Pregnancy</subject><subject>pregnant women</subject><subject>Scientific imaging</subject><subject>semiquantitative culture</subject><subject>Streptococcus</subject><subject>Streptococcus infections</subject><subject>Vagina</subject><subject>vaginal microbiota</subject><subject>Womens health</subject><subject>Yeasts</subject><issn>2076-0817</issn><issn>2076-0817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNplks9u1DAQxiMEolXpA3BBlrhwWfCfOE4uSLAqpdJKVGLhao2dSdar1F5sp6i8AK-Nt1uqFnzx2P7mN2P7q6qXjL4VoqPvdpA3YUSfGKMd7Vr6pDrmVDUL2jL19EF8VJ2mtKVltHS_fl4diYbVsmb8uPp9GUNGm901krNhKBEJA1mBzcGAddM0J2KjS6VaiWAE51Mm32F0HiayDFPw7hdkFzz56fKGnMcw78hH8jVH3OVgg7WOOE_yBsl642JP1tFdYcoY94UuI44evL15UT0bYEp4ejefVN8-na2XnxerL-cXyw-rha07kRemBobSGKRCATdKNX3TNMCUVIqa2gy9gl7UYAww6HsuKDKGDOqBYm1kK06qiwO3D7DVu9ILxBsdwOnbjRBHDTE7O6FWreKyllyKFmrBbScUVR3jKGlj-WAL6_2BtZvNFfYWfY4wPYI-PvFuo8dwrbtC4LIrgDd3gBh-zOVR9JVLFqcJPIY5aa6YajouGSvS1_9It2GO5Q9uVY1sGaeiqNhBZWNIKeJw3wyjeu8a_Z9rSs6rh7e4z_jrEfEHOLHB7Q</recordid><startdate>20220827</startdate><enddate>20220827</enddate><creator>Starc, Maja</creator><creator>Lučovnik, Miha</creator><creator>Eržen Vrlič, Petra</creator><creator>Jeverica, Samo</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-8373-8080</orcidid></search><sort><creationdate>20220827</creationdate><title>Protective Effect of Lactobacillus crispatus against Vaginal Colonization with Group B Streptococci in the Third Trimester of Pregnancy</title><author>Starc, Maja ; Lučovnik, Miha ; Eržen Vrlič, Petra ; Jeverica, Samo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-b4a1e5bbe037a2b776d666a175770b4bfd7ad34abba1add230e11e1a4f0e4b583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aerobic bacteria</topic><topic>Aerobic microorganisms</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Childbirth & labor</topic><topic>Colonization</topic><topic>Enrichment media</topic><topic>Gestational age</topic><topic>group B Streptococcus</topic><topic>Lactobacilli</topic><topic>Lactobacillus crispatus</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Neonates</topic><topic>Pregnancy</topic><topic>pregnant women</topic><topic>Scientific imaging</topic><topic>semiquantitative culture</topic><topic>Streptococcus</topic><topic>Streptococcus infections</topic><topic>Vagina</topic><topic>vaginal microbiota</topic><topic>Womens health</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Starc, Maja</creatorcontrib><creatorcontrib>Lučovnik, Miha</creatorcontrib><creatorcontrib>Eržen Vrlič, Petra</creatorcontrib><creatorcontrib>Jeverica, Samo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>Pathogens (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Starc, Maja</au><au>Lučovnik, Miha</au><au>Eržen Vrlič, Petra</au><au>Jeverica, Samo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective Effect of Lactobacillus crispatus against Vaginal Colonization with Group B Streptococci in the Third Trimester of Pregnancy</atitle><jtitle>Pathogens (Basel)</jtitle><addtitle>Pathogens</addtitle><date>2022-08-27</date><risdate>2022</risdate><volume>11</volume><issue>9</issue><spage>980</spage><pages>980-</pages><issn>2076-0817</issn><eissn>2076-0817</eissn><abstract>Background: A normal vaginal microbiota may protect the vaginal mucosa from colonization by potentially pathogenic bacteria, including group B streptococci (GBS). The aim of this study was to investigate the association between colonization with GBS and the presence of specific vaginal microbiota isolated from vaginal swabs in the third trimester of pregnancy. Methods: A semiquantitative culture of 1860 vaginal swabs from consecutive pregnant women in their third trimester was analyzed. The dominant bacteria, including lactobacilli, were identified using MALDI-TOF mass spectrometry. An enrichment culture for GBS was performed on the swabs. GBS colonization correlated with the bacteria isolated at the same time. Results: Lactobacilluscrispatus was isolated in 27.5% of the cultures, followed by L. jensenii (13.9%), L. gasseri (12.6%), and L. iners (10.1%). The presence of lactobacilli as a group, and of L. crispatus, inversely correlated with GBS colonization (OR = 0.44 and OR = 0.5, respectively; both with p < 0.001). Other microorganisms, including Gardnerella vaginalis, mixed aerobic bacteria and yeasts, were not associated with GBS colonization. Conclusions: Lactobacilli, especially L. crispatus, may prevent GBS colonization in pregnancy. Maintaining a normal vaginal microbiota could be an effective method for the antibiotic-free prevention of invasive GBS infections in neonates.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36145412</pmid><doi>10.3390/pathogens11090980</doi><orcidid>https://orcid.org/0000-0002-8373-8080</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Aerobic bacteria Aerobic microorganisms Antibiotics Bacteria Childbirth & labor Colonization Enrichment media Gestational age group B Streptococcus Lactobacilli Lactobacillus crispatus Mass spectrometry Mass spectroscopy Microbiota Microorganisms Neonates Pregnancy pregnant women Scientific imaging semiquantitative culture Streptococcus Streptococcus infections Vagina vaginal microbiota Womens health Yeasts |
title | Protective Effect of Lactobacillus crispatus against Vaginal Colonization with Group B Streptococci in the Third Trimester of Pregnancy |
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