Dietary Lactobacillus delbrueckii Affects Ileal Bacterial Composition and Circadian Rhythms in Pigs

Intestinal bacteria, synchronized with diet and feeding time, exhibit circadian rhythms and anticipate host gut function; however the effect of dietary probiotics on gut bacterial diurnal rhythms remains obscure. In this study, bacteria were sequenced at 6 Zeitgeber times (ZT) from a pig model of il...

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Bibliographic Details
Published in:Animals (Basel) 2024-01, Vol.14 (3), p.412
Main Authors: Luo, Wenxin, Yin, Zhangzheng, Zhang, Mingliang, Huang, Xingguo, Yin, Jie
Format: Article
Language:English
Subjects:
Bacteria
Bar codes
Catheters
Circadian rhythm
circadian rhythms
Diet
Genetic testing
Genomes
Hogs
ileal bacteria
L. delbrueckii
Metabolism
Microbiota
Nutrients
Nutrition
pigs
Probiotics
Small intestine
Software
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Summary:Intestinal bacteria, synchronized with diet and feeding time, exhibit circadian rhythms and anticipate host gut function; however the effect of dietary probiotics on gut bacterial diurnal rhythms remains obscure. In this study, bacteria were sequenced at 6 Zeitgeber times (ZT) from a pig model of ileal T-shaped fistula to test ileal bacterial composition and circadian rhythms after administration. The results showed that dietary enhanced ileal bacterial α-diversity at Zeitgeber time (ZT) 16, evidenced by an increased Simpson index compared with control pigs. At the phylum level, Firmicutes was identified as the largest phyla represented in pigs, but dietary only increased the abundance of Tenericutes at ZT16. At the genus level, 11/100 genera (i.e., , , , , , , , , , , and ) were markedly differentiated in -fed pigs and the effect was rhythmicity-dependent. Meanwhile, dietary affected six pathways of bacterial functions, such as membrane transport, metabolism of cofactors and vitamins, cell motility, the endocrine system, signaling molecules and interaction, and the nervous system. Cosinor analysis was conducted to test bacterial circadian rhythm in pigs, while no significant circadian rhythm in bacterial α-diversity and phyla composition was observed. , , and exhibited significant rhythmic fluctuation in the control pigs, which was disturbed by probiotic exposure. In addition, dietary affected circadian rhythms in ileal , , , and abundances. Dietary affected both ileal bacterial composition and circadian rhythms, which might further regulate gut function and host metabolism in pigs.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani14030412