Nanoencapsulation improves the protective effects of a nitric oxide donor on drought-stressed Heliocarpus popayanensis seedlings

• Published in: Ecotoxicology and environmental safety 2021-12, Vol.225, p.112713-112713, Article 112713
• Main Authors: do Carmo, Giovanna Camargo, Iastrenski, Lorena Felix, Debiasi, Tatiane Viegas, da Silva, Rafael Caetano, Gomes, Diego Genuário, Pelegrino, Milena Trevisan, Bianchini, Edmilson, Stolf-Moreira, Renata, Pimenta, José Antonio, Seabra, Amedea Barozzi, Oliveira, Halley Caixeta
• Format: Article
• Language: English
• Subjects: Chitosan; Nanoparticles; Photosynthesis; Root hairs; S-nitrosothiol; Water deficit
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112713

Despite the important role played by nitric oxide (NO) in plants subjected to abiotic stress, NO donors application to induce drought tolerance in neotropical tree seedlings has not yet been tested. It is also worth investigating whether NO bioactivity in drought-stressed seedlings could be potentiated by NO donors nanoencapsulation. The aim of the current study is to evaluate the effects of chitosan nanoparticles (NPs) containing S-nitroso-mercaptosuccinic acid (S-nitroso-MSA) on drought-stressed seedlings of neotropical tree species Heliocarpus popayanensis Kunth in comparison to free NO donor and NPs loaded with non-nitrosated MSA. Nanoencapsulation slowed down NO release from S-nitroso-MSA, and nanoencapsulated S-nitroso-MSA yielded 2- and 1.6-fold higher S-nitrosothiol levels in H. popayanensis roots and leaves, respectively, than the free NO donor. S-nitroso-MSA has prevented drought-induced CO2 assimilation inhibition, regardless of nanoencapsulation, but the nanoencapsulated NO donor has induced earlier ameliorative effect. Both NO and MSA have decreased oxidative stress in H. popayanensis roots, but this effect was not associated with antioxidant enzyme induction, with higher seedling biomass, or with proline and glycine betaine accumulation. Nanoencapsulated S-nitroso-MSA was the only formulation capable of increasing leaf relative water content in drought-stressed plants (from 32.3% to 60.5%). In addition, it induced root hair formation (increase by 36.6% in comparison to well-hydrated plants). Overall, results have evidenced that nanoencapsulation was capable of improving the protective effect of S-nitroso-MSA on H. popayanensis seedlings subjected to drought stress, a fact that highlighted the potential application of NO-releasing NPs to obtain drought-tolerant tree seedlings for reforestation programs. [Display omitted] •S-nitroso-MSA protected neotropical tree seedlings from drought stress.•S-nitroso-MSA nanoencapsulation improved NO availability to drought-stressed plants.•Nanoencapsulation boosted NO-induced improvement of photosynthesis.•Only the nanoencapsulated NO donor avoided leaf water content decrease.•The nanoencapsulated NO donor has induced unique changes in root morphology.