Horizontal transmission and recombination of Wolbachia in the butterfly tribe Aeromachini Tutt, 1906 (Lepidoptera: Hesperiidae)

• Published in: G3 : genes - genomes - genetics 2021-09, Vol.11 (9)
• Main Authors: Zhao, Zimiao, Zhu, Jianqing, Hoffmann, Ary A, Cao, Lijun, Shen, Li, Fang, Jie, Ma, Shuojia, Liu, Qunxiu, Yu, Weidong, Tang, Liying, Wang, Yongqiang, Jiang, Weibin
• Format: Article
• Language: English
• Subjects: Investigation
• ISSN: 2160-1836; 2160-1836
• DOI: 10.1093/g3journal/jkab221

Wolbachia is arguably one of the most ubiquitous heritable symbionts among insects and understanding its transmission dynamics is crucial for understanding why it is so common. While previous research has studied the transmission pathways of Wolbachia in several insect lineages including Lepidoptera, this study takes advantage of data collected from the lepidopteran tribe Aeromachini in an effort to assess patterns of transmission. Twenty-one of the 46 species of Aeromachini species were infected with Wolbachia. Overall, 25% (31/125) of Aeromachini specimens tested were Wolbachia positive. All Wolbachia strains were species-specific except for the wJho strain which appeared to be shared by three host species with a sympatric distribution based on a cophylogenetic comparison between Wolbachia and the Aeromachini species. Two tests of phylogenetic congruence did not find any evidence for cospeciation between Wolbachia strains and their butterfly hosts. The cophylogenetic comparison, divergence time estimation, and Wolbachia recombination analysis revealed that Wolbachia acquisition in Aeromachini appears to have mainly occurred mainly through horizontal transmission rather than codivergence.