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Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles

Gold nanoclusters (Au NCs) are attractive luminescent nanoprobes for biomedical applications. In vivo biosensing and bioimaging requires the delivery of the Au NCs into subcellular compartments. In this view, we explore here the possible encapsulation of ultra-small-sized red and blue emitting Au NC...

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Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2022-11, Vol.12 (21), p.3875
Main Authors: Chiechio, Regina M., Ducarre, Solène, Marets, Célia, Dupont, Aurélien, Even-Hernandez, Pascale, Pinson, Xavier, Dutertre, Stéphanie, Artzner, Franck, Musumeci, Paolo, Ravel, Célia, Faro, Maria Jose Lo, Marchi, Valérie
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Ducarre, Solène
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Musumeci, Paolo
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description Gold nanoclusters (Au NCs) are attractive luminescent nanoprobes for biomedical applications. In vivo biosensing and bioimaging requires the delivery of the Au NCs into subcellular compartments. In this view, we explore here the possible encapsulation of ultra-small-sized red and blue emitting Au NCs into liposomes of various sizes and chemical compositions. Different methods were investigated to prepare vesicles containing Au NCs in their lumen. The efficiency of the process was correlated to the structural and morphological aspect of the Au NCs’ encapsulating vesicles thanks to complementary analyses by SAXS, cryo-TEM, and confocal microscopy techniques. Cell-like-sized vesicles (GUVs) encapsulating red or blue Au NCs were successfully obtained by an innovative method using emulsion phase transfer. Furthermore, exosome-like-sized vesicles (LUVs) containing Au NCs were obtained with an encapsulation yield of 40%, as estimated from ICP-MS.
doi_str_mv 10.3390/nano12213875
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subjects Analysis
Aqueous solutions
Bioengineering
Biomedical materials
Biosensors
Chemical composition
Chemical Sciences
Confocal microscopy
Electronic equipment and supplies
Encapsulation
Gold
Gold compounds
gold nanoclusters
Identification and classification
Imaging
Life Sciences
Lipids
Material chemistry
Mechanical properties
Medical imaging
Microscopy
Nanoclusters
Nanoparticles
Optical properties
Plastic embedment
Quantum dots
Sucrose
Surface chemistry
Vesicles
Viscosity
title Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles
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