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Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles
Gold nanoclusters (Au NCs) are attractive luminescent nanoprobes for biomedical applications. In vivo biosensing and bioimaging requires the delivery of the Au NCs into subcellular compartments. In this view, we explore here the possible encapsulation of ultra-small-sized red and blue emitting Au NC...
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Published in: | Nanomaterials (Basel, Switzerland) Switzerland), 2022-11, Vol.12 (21), p.3875 |
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creator | Chiechio, Regina M. Ducarre, Solène Marets, Célia Dupont, Aurélien Even-Hernandez, Pascale Pinson, Xavier Dutertre, Stéphanie Artzner, Franck Musumeci, Paolo Ravel, Célia Faro, Maria Jose Lo Marchi, Valérie |
description | Gold nanoclusters (Au NCs) are attractive luminescent nanoprobes for biomedical applications. In vivo biosensing and bioimaging requires the delivery of the Au NCs into subcellular compartments. In this view, we explore here the possible encapsulation of ultra-small-sized red and blue emitting Au NCs into liposomes of various sizes and chemical compositions. Different methods were investigated to prepare vesicles containing Au NCs in their lumen. The efficiency of the process was correlated to the structural and morphological aspect of the Au NCs’ encapsulating vesicles thanks to complementary analyses by SAXS, cryo-TEM, and confocal microscopy techniques. Cell-like-sized vesicles (GUVs) encapsulating red or blue Au NCs were successfully obtained by an innovative method using emulsion phase transfer. Furthermore, exosome-like-sized vesicles (LUVs) containing Au NCs were obtained with an encapsulation yield of 40%, as estimated from ICP-MS. |
doi_str_mv | 10.3390/nano12213875 |
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subjects | Analysis Aqueous solutions Bioengineering Biomedical materials Biosensors Chemical composition Chemical Sciences Confocal microscopy Electronic equipment and supplies Encapsulation Gold Gold compounds gold nanoclusters Identification and classification Imaging Life Sciences Lipids Material chemistry Mechanical properties Medical imaging Microscopy Nanoclusters Nanoparticles Optical properties Plastic embedment Quantum dots Sucrose Surface chemistry Vesicles Viscosity |
title | Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles |
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