Regulators of cell movement during development and regeneration in Drosophila

Cell migration is a fundamental cell biological process essential both for normal development and for tissue regeneration after damage. Cells can migrate individually or as a collective. To better understand the genetic requirements for collective migration, we expressed RNA interference (RNAi) agai...

Full description

Saved in:
Bibliographic Details
Published in:Open biology 2019-05, Vol.9 (5), p.180245-180245
Main Authors: Myat, Monn Monn, Louis, Dheveline, Mavrommatis, Andreas, Collins, Latoya, Mattis, Jamal, Ledru, Michelle, Verghese, Shilpi, Su, Tin Tin
Format: Article
Language:English
Subjects:
Animals
Cell Culture Techniques
cell migration
Cell Movement
Cells, Cultured
Diacylglycerol Kinase - genetics
drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins - genetics
MAP Kinase Signaling System
rac GTP-Binding Proteins - genetics
RAC2 GTP-Binding Protein
radiation
Regeneration
RNA Interference
salivary gland
Salivary Glands - cytology
Salivary Glands - embryology
Salivary Glands - metabolism
wing disc
Wings, Animal - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell migration is a fundamental cell biological process essential both for normal development and for tissue regeneration after damage. Cells can migrate individually or as a collective. To better understand the genetic requirements for collective migration, we expressed RNA interference (RNAi) against 30 genes in the Drosophila embryonic salivary gland cells that are known to migrate collectively. The genes were selected based on their effect on cell and membrane morphology, cytoskeleton and cell adhesion in cell culture-based screens or in Drosophila tissues other than salivary glands. Of these, eight disrupted salivary gland migration, targeting: Rac2, Rab35 and Rab40 GTPases, MAP kinase-activated kinase-2 (MAPk-AK2), RdgA diacylglycerol kinase, Cdk9, the PDSW subunit of NADH dehydrogenase (ND-PDSW) and actin regulator Enabled (Ena). The same RNAi lines were used to determine their effect during regeneration of X-ray-damaged larval wing discs. Cells translocate during this process, but it remained unknown whether they do so by directed cell divisions, by cell migration or both. We found that RNAi targeting Rac2, MAPk-AK2 and RdgA disrupted cell translocation during wing disc regeneration, but RNAi against Ena and ND-PDSW had little effect. We conclude that, in Drosophila, cell movements in development and regeneration have common as well as distinct genetic requirements.
ISSN:2046-2441
2046-2441
DOI:10.1098/rsob.180245