A comprehensive analysis of the role of QPRT in breast cancer

To explore the clinical role of QPRT in breast cancer. The gene expression, methylation levels and prognostic value of QPRT in breast cancer was analyzed using TCGA data. Validation was performed using the data from GEO dataset and TNMPLOT database. Meta analysis method was used to pool the survival...

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Bibliographic Details
Published in:Scientific reports 2023-09, Vol.13 (1), p.15414-15414, Article 15414
Main Authors: Yan, Yiqing, Li, Lun, Wang, Zixin, Pang, Jian, Guan, Xinyu, Yuan, Yunchang, Xia, Zhenkun, Yi, Wenjun
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
631/67/1347
631/67/1857
631/67/580
AKT protein
Anthracycline
Breast cancer
Cell cycle
DNA methylation
Drug resistance
ErbB-2 protein
Gene expression
Humanities and Social Sciences
Immunosuppressive agents
Macrophages
Metastases
multidisciplinary
Science
Science (multidisciplinary)
Signal transduction
Wnt protein
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Summary:To explore the clinical role of QPRT in breast cancer. The gene expression, methylation levels and prognostic value of QPRT in breast cancer was analyzed using TCGA data. Validation was performed using the data from GEO dataset and TNMPLOT database. Meta analysis method was used to pool the survival data for QPRT. The predictive values of QPRT for different drugs were retrieved from the ROC plot. The expression differences of QPRT in acquired drug-resistant and sensitive cell lines were analyzed using GEO datasets. GO and KEGG enrichment analysis were conducted for those genes which were highly co-expressed with QPRT in tissue based on TCGA data and which changed after QPRT knockdown. Timer2.0 was utilized to explore the correlation between QPRT and immune cells infiltration, and the Human Protein Atlas was used to analyse QPRT’s single-cell sequencing data across different human tissues. The expression of QPRT in different types of macrophages, and the expression of QPRT were analysed after coculturing HER2+ breast cancer cells with macrophages. Additionally, TargetScan, Comparative Toxicogenomics and the connectivity map were used to research miRNAs and drugs that could regulate QPRT expression. Cytoscape was used to map the interaction networks between QPRT and other proteins. QPRT was highly expressed in breast cancer tissue and highly expressed in HER2+ breast cancer patients ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-42566-4