Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes

• Published in: Scientific reports 2017-03, Vol.7 (1), p.47-14, Article 47
• Main Authors: de Gonzalo-Calvo, D., van der Meer, R. W., Rijzewijk, L. J., Smit, J. W. A., Revuelta-Lopez, E., Nasarre, L., Escola-Gil, J. C., Lamb, H. J., Llorente-Cortes, V.
• Format: Article
• Language: English
• Subjects: 13; 38; 692/4019/592; 692/53/2423; Aged; Animal models; Animals; Biomarkers - blood; Cardiomyocytes; Cardiomyopathy; Cardiovascular diseases; Coronary artery disease; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diabetic Cardiomyopathies - blood; Diabetic Cardiomyopathies - etiology; Diet, High-Fat; Exosomes; Heart diseases; High fat diet; Humanities and Social Sciences; Humans; Ischemia; Male; Mice; Mice, Inbred C57BL; MicroRNAs; MicroRNAs - blood; Middle Aged; miRNA; multidisciplinary; Myocardium - pathology; Myocytes, Cardiac - pathology; Proton Magnetic Resonance Spectroscopy; Rodents; Science; Science (multidisciplinary); Steatosis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-00070-6

Using in vitro , in vivo and patient-based approaches, we investigated the potential of circulating microRNAs (miRNAs) as surrogate biomarkers of myocardial steatosis, a hallmark of diabetic cardiomyopathy. We analysed the cardiomyocyte-enriched miRNA signature in serum from patients with well-controlled type 2 diabetes and with verified absence of structural heart disease or inducible ischemia, and control volunteers of the same age range and BMI (N = 86), in serum from a high-fat diet-fed murine model, and in exosomes from lipid-loaded HL-1 cardiomyocytes. Circulating miR-1 and miR-133a levels were robustly associated with myocardial steatosis in type 2 diabetes patients, independently of confounding factors in both linear and logistic regression analyses ( P