Loading…
Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy
Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and...
Saved in:
| Published in: | Scientific reports 2024-04, Vol.14 (1), p.8515-8515, Article 8515 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | cdi_FETCH-LOGICAL-c436t-5be735cd6533a6d776a75fc0a44eb1bd1d678cf2a4d63d9f4160fe3377a3c4073 |
| container_end_page | 8515 |
| container_issue | 1 |
| container_start_page | 8515 |
| container_title | Scientific reports |
| container_volume | 14 |
| creator | Hendawy, Seham H. M. Alzan, Heba F. Abdel-Ghany, Hoda S. M. Suarez, Carlos E. Kamel, Gihan |
| description | Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and acaricide-resistant tick strains, necessitates the development of novel ameliorative tick control strategies such as vaccines. The synchrotron-based Fourier transform infrared micro-spectroscopy (SR-FTIR) is a bioanalytical microprobe capable of exploring the molecular chemistry within microstructures at a cellular or subcellular level and is considered as a nondestructive analytical approach for biological specimens. In this study, SR-FTIR analysis was able to explore a qualitative and semi-quantitative biochemical composition of gut and salivary glands of
Hyalomma dromedarii
(
H. dromedarii
) tick detecting differences in the biochemical composition of both tissues. A notable observation regarding Amide I secondary structure protein profile was the higher ratio of aggregated strands in salivary gland and beta turns in gut tissues. Regarding the lipid profile, there was a higher intensity of lipid regions in gut tissue when compared to salivary glands. This detailed information on the biochemical compositions of tick tissues could assist in selecting vaccine and/or control candidates. Altogether, these findings confirmed SR-FTIR spectroscopy as a tool for detecting differences in the biochemical composition of
H. dromedarii
salivary glands and gut tissues. This approach could potentially be extended to the analysis of other ticks that are vectors of important diseases such as babesiosis and theileriosis. |
| doi_str_mv | 10.1038/s41598-024-59165-6 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_78f7a1974cc04ff7a4cad6a3a4e0c059</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_78f7a1974cc04ff7a4cad6a3a4e0c059</doaj_id><sourcerecordid>3037692004</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-5be735cd6533a6d776a75fc0a44eb1bd1d678cf2a4d63d9f4160fe3377a3c4073</originalsourceid><addsrcrecordid>eNp9kU9P3DAQxaOqqCDgC_RQWeqll7T-7-TYolJWQkICerZmbSf1KllvPQnSfvuaDVDUQ33xyP7N8_i9qnrP6GdGRfMFJVNtU1Mua9UyrWr9pjrhVKqaC87fvqqPq3PEDS1L8Vay9l11LBpNWyn5STV8i8n9CmN0MBDYwrDHiCR15GoPQxpHID6nMXjIMRKEIT5A3pN-gK3HwnvSzxOZIuIckMwYtz25u60v71e3pGjmVOMuuCkndGm3P6uOOhgwnD_tp9XPy-_3F1f19c2P1cXX69pJoadarYMRynmthADtjdFgVOcoSBnWbO2Z16ZxHQfptfBtJ5mmXRDCGBBOUiNOq9Wi6xNs7C7HsQxtE0R7OEi5t5Cn6IZgTdMZYK2RzlHZlVo68BoEyEAdVW3R-rRo7XL6XT452TGiC0NxIKQZrShhSEm1kQX9-A-6SXMunh4oo1tO6SPFF6q4g5hD9zIgo_YxWrtEa0u09hCt1aXpw5P0vC5pvLQ8B1kAsQBYrrZ9yH_f_o_sH6TDrtw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3037692004</pqid></control><display><type>article</type><title>Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy</title><source>Publicly Available Content Database</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Hendawy, Seham H. M. ; Alzan, Heba F. ; Abdel-Ghany, Hoda S. M. ; Suarez, Carlos E. ; Kamel, Gihan</creator><creatorcontrib>Hendawy, Seham H. M. ; Alzan, Heba F. ; Abdel-Ghany, Hoda S. M. ; Suarez, Carlos E. ; Kamel, Gihan</creatorcontrib><description>Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and acaricide-resistant tick strains, necessitates the development of novel ameliorative tick control strategies such as vaccines. The synchrotron-based Fourier transform infrared micro-spectroscopy (SR-FTIR) is a bioanalytical microprobe capable of exploring the molecular chemistry within microstructures at a cellular or subcellular level and is considered as a nondestructive analytical approach for biological specimens. In this study, SR-FTIR analysis was able to explore a qualitative and semi-quantitative biochemical composition of gut and salivary glands of
Hyalomma dromedarii
(
H. dromedarii
) tick detecting differences in the biochemical composition of both tissues. A notable observation regarding Amide I secondary structure protein profile was the higher ratio of aggregated strands in salivary gland and beta turns in gut tissues. Regarding the lipid profile, there was a higher intensity of lipid regions in gut tissue when compared to salivary glands. This detailed information on the biochemical compositions of tick tissues could assist in selecting vaccine and/or control candidates. Altogether, these findings confirmed SR-FTIR spectroscopy as a tool for detecting differences in the biochemical composition of
H. dromedarii
salivary glands and gut tissues. This approach could potentially be extended to the analysis of other ticks that are vectors of important diseases such as babesiosis and theileriosis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-024-59165-6</identifier><identifier>PMID: 38609442</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/2204 ; 631/1647/245 ; Acaricides ; Animals ; Arachnids ; Babesiosis ; Biochemical analysis ; Biochemical composition ; Climate change ; Exocrine glands ; Fourier transforms ; Humanities and Social Sciences ; Humans ; Hyalomma dromedarii ; Infrared spectroscopy ; Ixodidae ; Lipids ; multidisciplinary ; Protein structure ; Salivary gland ; Salivary Glands ; Science ; Science (multidisciplinary) ; Secondary structure ; Spectroscopy ; Spectroscopy, Fourier Transform Infrared ; Synapsins ; Theileriosis ; Tick-borne diseases ; Tissues ; Vaccines ; Vectors</subject><ispartof>Scientific reports, 2024-04, Vol.14 (1), p.8515-8515, Article 8515</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c436t-5be735cd6533a6d776a75fc0a44eb1bd1d678cf2a4d63d9f4160fe3377a3c4073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3037692004/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3037692004?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38609442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hendawy, Seham H. M.</creatorcontrib><creatorcontrib>Alzan, Heba F.</creatorcontrib><creatorcontrib>Abdel-Ghany, Hoda S. M.</creatorcontrib><creatorcontrib>Suarez, Carlos E.</creatorcontrib><creatorcontrib>Kamel, Gihan</creatorcontrib><title>Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and acaricide-resistant tick strains, necessitates the development of novel ameliorative tick control strategies such as vaccines. The synchrotron-based Fourier transform infrared micro-spectroscopy (SR-FTIR) is a bioanalytical microprobe capable of exploring the molecular chemistry within microstructures at a cellular or subcellular level and is considered as a nondestructive analytical approach for biological specimens. In this study, SR-FTIR analysis was able to explore a qualitative and semi-quantitative biochemical composition of gut and salivary glands of
Hyalomma dromedarii
(
H. dromedarii
) tick detecting differences in the biochemical composition of both tissues. A notable observation regarding Amide I secondary structure protein profile was the higher ratio of aggregated strands in salivary gland and beta turns in gut tissues. Regarding the lipid profile, there was a higher intensity of lipid regions in gut tissue when compared to salivary glands. This detailed information on the biochemical compositions of tick tissues could assist in selecting vaccine and/or control candidates. Altogether, these findings confirmed SR-FTIR spectroscopy as a tool for detecting differences in the biochemical composition of
H. dromedarii
salivary glands and gut tissues. This approach could potentially be extended to the analysis of other ticks that are vectors of important diseases such as babesiosis and theileriosis.</description><subject>631/1647/2204</subject><subject>631/1647/245</subject><subject>Acaricides</subject><subject>Animals</subject><subject>Arachnids</subject><subject>Babesiosis</subject><subject>Biochemical analysis</subject><subject>Biochemical composition</subject><subject>Climate change</subject><subject>Exocrine glands</subject><subject>Fourier transforms</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hyalomma dromedarii</subject><subject>Infrared spectroscopy</subject><subject>Ixodidae</subject><subject>Lipids</subject><subject>multidisciplinary</subject><subject>Protein structure</subject><subject>Salivary gland</subject><subject>Salivary Glands</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Secondary structure</subject><subject>Spectroscopy</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Synapsins</subject><subject>Theileriosis</subject><subject>Tick-borne diseases</subject><subject>Tissues</subject><subject>Vaccines</subject><subject>Vectors</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kU9P3DAQxaOqqCDgC_RQWeqll7T-7-TYolJWQkICerZmbSf1KllvPQnSfvuaDVDUQ33xyP7N8_i9qnrP6GdGRfMFJVNtU1Mua9UyrWr9pjrhVKqaC87fvqqPq3PEDS1L8Vay9l11LBpNWyn5STV8i8n9CmN0MBDYwrDHiCR15GoPQxpHID6nMXjIMRKEIT5A3pN-gK3HwnvSzxOZIuIckMwYtz25u60v71e3pGjmVOMuuCkndGm3P6uOOhgwnD_tp9XPy-_3F1f19c2P1cXX69pJoadarYMRynmthADtjdFgVOcoSBnWbO2Z16ZxHQfptfBtJ5mmXRDCGBBOUiNOq9Wi6xNs7C7HsQxtE0R7OEi5t5Cn6IZgTdMZYK2RzlHZlVo68BoEyEAdVW3R-rRo7XL6XT452TGiC0NxIKQZrShhSEm1kQX9-A-6SXMunh4oo1tO6SPFF6q4g5hD9zIgo_YxWrtEa0u09hCt1aXpw5P0vC5pvLQ8B1kAsQBYrrZ9yH_f_o_sH6TDrtw</recordid><startdate>20240412</startdate><enddate>20240412</enddate><creator>Hendawy, Seham H. M.</creator><creator>Alzan, Heba F.</creator><creator>Abdel-Ghany, Hoda S. M.</creator><creator>Suarez, Carlos E.</creator><creator>Kamel, Gihan</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20240412</creationdate><title>Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy</title><author>Hendawy, Seham H. M. ; Alzan, Heba F. ; Abdel-Ghany, Hoda S. M. ; Suarez, Carlos E. ; Kamel, Gihan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-5be735cd6533a6d776a75fc0a44eb1bd1d678cf2a4d63d9f4160fe3377a3c4073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>631/1647/2204</topic><topic>631/1647/245</topic><topic>Acaricides</topic><topic>Animals</topic><topic>Arachnids</topic><topic>Babesiosis</topic><topic>Biochemical analysis</topic><topic>Biochemical composition</topic><topic>Climate change</topic><topic>Exocrine glands</topic><topic>Fourier transforms</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Hyalomma dromedarii</topic><topic>Infrared spectroscopy</topic><topic>Ixodidae</topic><topic>Lipids</topic><topic>multidisciplinary</topic><topic>Protein structure</topic><topic>Salivary gland</topic><topic>Salivary Glands</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Secondary structure</topic><topic>Spectroscopy</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Synapsins</topic><topic>Theileriosis</topic><topic>Tick-borne diseases</topic><topic>Tissues</topic><topic>Vaccines</topic><topic>Vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hendawy, Seham H. M.</creatorcontrib><creatorcontrib>Alzan, Heba F.</creatorcontrib><creatorcontrib>Abdel-Ghany, Hoda S. M.</creatorcontrib><creatorcontrib>Suarez, Carlos E.</creatorcontrib><creatorcontrib>Kamel, Gihan</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Directory of Open Access Journals</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hendawy, Seham H. M.</au><au>Alzan, Heba F.</au><au>Abdel-Ghany, Hoda S. M.</au><au>Suarez, Carlos E.</au><au>Kamel, Gihan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2024-04-12</date><risdate>2024</risdate><volume>14</volume><issue>1</issue><spage>8515</spage><epage>8515</epage><pages>8515-8515</pages><artnum>8515</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and acaricide-resistant tick strains, necessitates the development of novel ameliorative tick control strategies such as vaccines. The synchrotron-based Fourier transform infrared micro-spectroscopy (SR-FTIR) is a bioanalytical microprobe capable of exploring the molecular chemistry within microstructures at a cellular or subcellular level and is considered as a nondestructive analytical approach for biological specimens. In this study, SR-FTIR analysis was able to explore a qualitative and semi-quantitative biochemical composition of gut and salivary glands of
Hyalomma dromedarii
(
H. dromedarii
) tick detecting differences in the biochemical composition of both tissues. A notable observation regarding Amide I secondary structure protein profile was the higher ratio of aggregated strands in salivary gland and beta turns in gut tissues. Regarding the lipid profile, there was a higher intensity of lipid regions in gut tissue when compared to salivary glands. This detailed information on the biochemical compositions of tick tissues could assist in selecting vaccine and/or control candidates. Altogether, these findings confirmed SR-FTIR spectroscopy as a tool for detecting differences in the biochemical composition of
H. dromedarii
salivary glands and gut tissues. This approach could potentially be extended to the analysis of other ticks that are vectors of important diseases such as babesiosis and theileriosis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>38609442</pmid><doi>10.1038/s41598-024-59165-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2024-04, Vol.14 (1), p.8515-8515, Article 8515 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_78f7a1974cc04ff7a4cad6a3a4e0c059 |
| source | Publicly Available Content Database; PubMed Central; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 631/1647/2204 631/1647/245 Acaricides Animals Arachnids Babesiosis Biochemical analysis Biochemical composition Climate change Exocrine glands Fourier transforms Humanities and Social Sciences Humans Hyalomma dromedarii Infrared spectroscopy Ixodidae Lipids multidisciplinary Protein structure Salivary gland Salivary Glands Science Science (multidisciplinary) Secondary structure Spectroscopy Spectroscopy, Fourier Transform Infrared Synapsins Theileriosis Tick-borne diseases Tissues Vaccines Vectors |
| title | Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T19%3A48%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biochemical%20analysis%20of%20Hyalomma%20dromedarii%20salivary%20glands%20and%20gut%20tissues%20using%20SR-FTIR%20micro-spectroscopy&rft.jtitle=Scientific%20reports&rft.au=Hendawy,%20Seham%20H.%20M.&rft.date=2024-04-12&rft.volume=14&rft.issue=1&rft.spage=8515&rft.epage=8515&rft.pages=8515-8515&rft.artnum=8515&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-024-59165-6&rft_dat=%3Cproquest_doaj_%3E3037692004%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c436t-5be735cd6533a6d776a75fc0a44eb1bd1d678cf2a4d63d9f4160fe3377a3c4073%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3037692004&rft_id=info:pmid/38609442&rfr_iscdi=true |