Pooling isolates to address the diversity in antimicrobial susceptibility of Pseudomonas aeruginosa in cystic fibrosis

People with cystic fibrosis (pwCF) often suffer from chronic lung infections with . While antibiotics are still commonly used to treat infections, there is a high discordance between and antibiotic efficacy, which contributes to suboptimal antibiotic therapy. In the present study, we found that isol...

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Bibliographic Details
Published in:Microbiology spectrum 2023-12, Vol.11 (6), p.e0044923-e0044923
Main Authors: Van den Bossche, Sara, Abatih, Emmanuel, Grassi, Lucia, De Broe, Emma, Rigole, Petra, Boelens, Jerina, Van Caenegem, Joris, Verhasselt, Bruno, Janssens, Iris, Van Braeckel, Eva, Versmessen, Nick, Cools, Piet, Coenye, Tom, Crabbé, Aurélie
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
antibiotic susceptibility testing
Clinical Microbiology
cystic fibrosis
Cystic Fibrosis - drug therapy
diversity
Humans
Microbial Sensitivity Tests
minimal inhibitory concentration
pooling isolates
Pseudomonas aeruginosa
Pseudomonas Infections - drug therapy
Research Article
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Summary:People with cystic fibrosis (pwCF) often suffer from chronic lung infections with . While antibiotics are still commonly used to treat infections, there is a high discordance between and antibiotic efficacy, which contributes to suboptimal antibiotic therapy. In the present study, we found that isolates from the same sputum sample had highly diverse antibiotic resistance profiles [based on the minimal inhibitory concentration (MIC)], which may explain the reported discrepancy between and antibiotic efficacy. Through systematic analysis, we report that pooling nine isolates per sputum sample significantly decreased intrasample diversity in MIC and influenced clinical interpretation of antibiotic susceptibility tests compared to single isolate testing. Hence, pooling of isolates may offer a solution to obtain a consistent MIC test result and could lead to optimizing antibiotic therapy in pwCF and other infectious diseases where diversity in antibiotic resistance is observed.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.00449-23