Purification and thrombolytic effects in vivo of recombination Nattokinase on carrageenan-induced tail thrombosis in a rat model

Nattokinase is a serine protease with fibrinolytic activity and has been proven clinical efficacy and safety for human use by the oral route. In this study, conducted purified recombinant nattokinase from Bacillus subtillis DB104 strain and thrombolytic effects of nattokinase have been observed in v...

Full description

Saved in:
Bibliographic Details
Published in:Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Online) 2020-08, Vol.8 (1), p.82-91
Main Authors: Tuan, Tran Quoc, Anh, Dinh Thi Lan, Ai, Le Thi Thuy, Hiep, Dinh Minh, Dong, Tran Cat
Format: Article
Language:English
Subjects:
bacillus subtilis
carrageenan
purification
recombinant nattokinase
thrombosis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 91
container_issue 1
container_start_page 82
container_title Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Online)
container_volume 8
creator Tuan, Tran Quoc
Anh, Dinh Thi Lan
Ai, Le Thi Thuy
Hiep, Dinh Minh
Dong, Tran Cat
description Nattokinase is a serine protease with fibrinolytic activity and has been proven clinical efficacy and safety for human use by the oral route. In this study, conducted purified recombinant nattokinase from Bacillus subtillis DB104 strain and thrombolytic effects of nattokinase have been observed in vivo. The result of the enzyme purification by membrane filtration method for enzyme recovery efficiency is 82.45%. Then the enzyme was purified to Q-Sepharose ion exchange chromatography with a purification factor of 1.82 and at a yield of 66.91%. Enzyme activity was 5,457 FU/mg protein. With the carrageenan-induced tail thrombosis in a rat model, results were significantly reduced by 67.3 to 83.6% at oral doses and injected nattokinase compared with the control group at 48 h. At 72 hours the rate increased to 82-89% in the oral dose and up to 91% in the injected dose nattokinase and comparable to 90% for heparin-positive hepatitis.
doi_str_mv 10.46223/HCMCOUJS.tech.en.8.1.907.2018
format article
fullrecord <record><control><sourceid>doaj_cross</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_7926c9d63b97492fae69df04a459f764</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_7926c9d63b97492fae69df04a459f764</doaj_id><sourcerecordid>oai_doaj_org_article_7926c9d63b97492fae69df04a459f764</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1214-75a0a7f8d96454cc5fa19a82b20cc3512c79f7fc0d357853ddba517a9c4ba2973</originalsourceid><addsrcrecordid>eNo9kUtP3DAQgKOKSiDgP_jELcHPOL5UqlY8xaNSy9majG0wzdrICUjc-tNrdllO89J89uhrmhNGO9lzLk4vV7er-4fr393i8anzqRs61hmqO07Z8K054FrI1vR02NvlQtD95nienyml3DDOlD5o_v16LTFEhCXmRCA5sjyVvB7z9L5EJD4Ej8tMYiJv8S2THEjxWOcxbTfuYFny31rNntQSoRR49D5BamNyr-grEOK0o85xwwJSYCHr7Px01HwPMM3--DMeNg_nZ39Wl-3N_cXV6udNi_WnstUKKOgwONNLJRFVAGZg4COniEIxjtoEHZA6ofSghHMjKKbBoByBGy0Om6st12V4ti8lrqG82wzRbhq5PFoo9eTJW214j8b1YjRaGh7A98YFKkGq-kYvK-vHloUlz3Px4YvHqN3osTs99kOP9ckOltmqx37oEf8BLXKJ-Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Purification and thrombolytic effects in vivo of recombination Nattokinase on carrageenan-induced tail thrombosis in a rat model</title><source>Directory of Open Access Journals(OpenAccess)</source><creator>Tuan, Tran Quoc ; Anh, Dinh Thi Lan ; Ai, Le Thi Thuy ; Hiep, Dinh Minh ; Dong, Tran Cat</creator><creatorcontrib>Tuan, Tran Quoc ; Anh, Dinh Thi Lan ; Ai, Le Thi Thuy ; Hiep, Dinh Minh ; Dong, Tran Cat</creatorcontrib><description>Nattokinase is a serine protease with fibrinolytic activity and has been proven clinical efficacy and safety for human use by the oral route. In this study, conducted purified recombinant nattokinase from Bacillus subtillis DB104 strain and thrombolytic effects of nattokinase have been observed in vivo. The result of the enzyme purification by membrane filtration method for enzyme recovery efficiency is 82.45%. Then the enzyme was purified to Q-Sepharose ion exchange chromatography with a purification factor of 1.82 and at a yield of 66.91%. Enzyme activity was 5,457 FU/mg protein. With the carrageenan-induced tail thrombosis in a rat model, results were significantly reduced by 67.3 to 83.6% at oral doses and injected nattokinase compared with the control group at 48 h. At 72 hours the rate increased to 82-89% in the oral dose and up to 91% in the injected dose nattokinase and comparable to 90% for heparin-positive hepatitis.</description><identifier>ISSN: 2734-9330</identifier><identifier>EISSN: 2734-9608</identifier><identifier>DOI: 10.46223/HCMCOUJS.tech.en.8.1.907.2018</identifier><language>eng</language><publisher>HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE</publisher><subject>bacillus subtilis ; carrageenan ; purification ; recombinant nattokinase ; thrombosis</subject><ispartof>Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Online), 2020-08, Vol.8 (1), p.82-91</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,2096,27905,27906</link.rule.ids></links><search><creatorcontrib>Tuan, Tran Quoc</creatorcontrib><creatorcontrib>Anh, Dinh Thi Lan</creatorcontrib><creatorcontrib>Ai, Le Thi Thuy</creatorcontrib><creatorcontrib>Hiep, Dinh Minh</creatorcontrib><creatorcontrib>Dong, Tran Cat</creatorcontrib><title>Purification and thrombolytic effects in vivo of recombination Nattokinase on carrageenan-induced tail thrombosis in a rat model</title><title>Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Online)</title><description>Nattokinase is a serine protease with fibrinolytic activity and has been proven clinical efficacy and safety for human use by the oral route. In this study, conducted purified recombinant nattokinase from Bacillus subtillis DB104 strain and thrombolytic effects of nattokinase have been observed in vivo. The result of the enzyme purification by membrane filtration method for enzyme recovery efficiency is 82.45%. Then the enzyme was purified to Q-Sepharose ion exchange chromatography with a purification factor of 1.82 and at a yield of 66.91%. Enzyme activity was 5,457 FU/mg protein. With the carrageenan-induced tail thrombosis in a rat model, results were significantly reduced by 67.3 to 83.6% at oral doses and injected nattokinase compared with the control group at 48 h. At 72 hours the rate increased to 82-89% in the oral dose and up to 91% in the injected dose nattokinase and comparable to 90% for heparin-positive hepatitis.</description><subject>bacillus subtilis</subject><subject>carrageenan</subject><subject>purification</subject><subject>recombinant nattokinase</subject><subject>thrombosis</subject><issn>2734-9330</issn><issn>2734-9608</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNo9kUtP3DAQgKOKSiDgP_jELcHPOL5UqlY8xaNSy9majG0wzdrICUjc-tNrdllO89J89uhrmhNGO9lzLk4vV7er-4fr393i8anzqRs61hmqO07Z8K054FrI1vR02NvlQtD95nienyml3DDOlD5o_v16LTFEhCXmRCA5sjyVvB7z9L5EJD4Ej8tMYiJv8S2THEjxWOcxbTfuYFny31rNntQSoRR49D5BamNyr-grEOK0o85xwwJSYCHr7Px01HwPMM3--DMeNg_nZ39Wl-3N_cXV6udNi_WnstUKKOgwONNLJRFVAGZg4COniEIxjtoEHZA6ofSghHMjKKbBoByBGy0Om6st12V4ti8lrqG82wzRbhq5PFoo9eTJW214j8b1YjRaGh7A98YFKkGq-kYvK-vHloUlz3Px4YvHqN3osTs99kOP9ckOltmqx37oEf8BLXKJ-Q</recordid><startdate>20200817</startdate><enddate>20200817</enddate><creator>Tuan, Tran Quoc</creator><creator>Anh, Dinh Thi Lan</creator><creator>Ai, Le Thi Thuy</creator><creator>Hiep, Dinh Minh</creator><creator>Dong, Tran Cat</creator><general>HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>20200817</creationdate><title>Purification and thrombolytic effects in vivo of recombination Nattokinase on carrageenan-induced tail thrombosis in a rat model</title><author>Tuan, Tran Quoc ; Anh, Dinh Thi Lan ; Ai, Le Thi Thuy ; Hiep, Dinh Minh ; Dong, Tran Cat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1214-75a0a7f8d96454cc5fa19a82b20cc3512c79f7fc0d357853ddba517a9c4ba2973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>bacillus subtilis</topic><topic>carrageenan</topic><topic>purification</topic><topic>recombinant nattokinase</topic><topic>thrombosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tuan, Tran Quoc</creatorcontrib><creatorcontrib>Anh, Dinh Thi Lan</creatorcontrib><creatorcontrib>Ai, Le Thi Thuy</creatorcontrib><creatorcontrib>Hiep, Dinh Minh</creatorcontrib><creatorcontrib>Dong, Tran Cat</creatorcontrib><collection>CrossRef</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Online)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tuan, Tran Quoc</au><au>Anh, Dinh Thi Lan</au><au>Ai, Le Thi Thuy</au><au>Hiep, Dinh Minh</au><au>Dong, Tran Cat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and thrombolytic effects in vivo of recombination Nattokinase on carrageenan-induced tail thrombosis in a rat model</atitle><jtitle>Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Online)</jtitle><date>2020-08-17</date><risdate>2020</risdate><volume>8</volume><issue>1</issue><spage>82</spage><epage>91</epage><pages>82-91</pages><issn>2734-9330</issn><eissn>2734-9608</eissn><abstract>Nattokinase is a serine protease with fibrinolytic activity and has been proven clinical efficacy and safety for human use by the oral route. In this study, conducted purified recombinant nattokinase from Bacillus subtillis DB104 strain and thrombolytic effects of nattokinase have been observed in vivo. The result of the enzyme purification by membrane filtration method for enzyme recovery efficiency is 82.45%. Then the enzyme was purified to Q-Sepharose ion exchange chromatography with a purification factor of 1.82 and at a yield of 66.91%. Enzyme activity was 5,457 FU/mg protein. With the carrageenan-induced tail thrombosis in a rat model, results were significantly reduced by 67.3 to 83.6% at oral doses and injected nattokinase compared with the control group at 48 h. At 72 hours the rate increased to 82-89% in the oral dose and up to 91% in the injected dose nattokinase and comparable to 90% for heparin-positive hepatitis.</abstract><pub>HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE</pub><doi>10.46223/HCMCOUJS.tech.en.8.1.907.2018</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2734-9330
ispartof Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Online), 2020-08, Vol.8 (1), p.82-91
issn 2734-9330
2734-9608
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_7926c9d63b97492fae69df04a459f764
source Directory of Open Access Journals(OpenAccess)
subjects bacillus subtilis
carrageenan
purification
recombinant nattokinase
thrombosis
title Purification and thrombolytic effects in vivo of recombination Nattokinase on carrageenan-induced tail thrombosis in a rat model
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T14%3A08%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-doaj_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20and%20thrombolytic%20effects%20in%20vivo%20of%20recombination%20Nattokinase%20on%20carrageenan-induced%20tail%20thrombosis%20in%20a%20rat%20model&rft.jtitle=Ho%20Chi%20Minh%20City%20Open%20University%20Journal%20of%20Science%20-%20Engineering%20and%20Technology%20(Online)&rft.au=Tuan,%20Tran%20Quoc&rft.date=2020-08-17&rft.volume=8&rft.issue=1&rft.spage=82&rft.epage=91&rft.pages=82-91&rft.issn=2734-9330&rft.eissn=2734-9608&rft_id=info:doi/10.46223/HCMCOUJS.tech.en.8.1.907.2018&rft_dat=%3Cdoaj_cross%3Eoai_doaj_org_article_7926c9d63b97492fae69df04a459f764%3C/doaj_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c1214-75a0a7f8d96454cc5fa19a82b20cc3512c79f7fc0d357853ddba517a9c4ba2973%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true