Terpene Synthases in Rice Pan-Genome and Their Responses to Chilo suppressalis Larvae Infesting

Terpene synthase (TPS) catalyzes the synthesis of terpenes and plays an important role in plant defense. This study identified 45 genes (32 core genes and 13 variable genes) based on the high-quality rice gene-based pan-genome. This indicates limitations in gene studies based on a single reference g...

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Bibliographic Details
Published in:Frontiers in plant science 2022-05, Vol.13, p.905982-905982
Main Authors: Sun, Yang, Zhang, Pei-Tao, Kou, Dou-Rong, Han, Yang-Chun, Fang, Ji-Chao, Ni, Jiang-Ping, Jiang, Bin, Wang, Xu, Zhang, Yong-Jun, Wang, Wei, Kong, Xiang-Dong
Format: Article
Language:English
Subjects:
Chilo suppressali
differentially expressed
Plant Science
rice pan-genome
selection pressure
structure variation
terpene synthase
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Summary:Terpene synthase (TPS) catalyzes the synthesis of terpenes and plays an important role in plant defense. This study identified 45 genes (32 core genes and 13 variable genes) based on the high-quality rice gene-based pan-genome. This indicates limitations in gene studies based on a single reference genome. In the present study, through collinearity between multiple rice genomes, one gene absent in the reference (Nipponbare) genome was found and two genes in the reference genome were found to have atypical structures, which would have been ignored in single genome analysis. genes were divided into five groups and TPS-b was lost according to the phylogenetic tree. in TPS-c and TPS-g were all core genes indicating these two groups were stable during domestication. In addition, through the analysis of transcriptome data, some structural variations were found to affect the expression of genes. Through the Ka/Ks calculation of genes, we found that different genes were under different selection pressure during domestication; for example, and experienced stronger positive selection than the other genes. After larvae infesting, 25 differentially expressed genes were identified, which are involved in the diterpene phytoalexins precursors biosynthesis and ent-kaurene biosynthesis pathways. Overall, the present study conducted a bioinformatics analysis of genes using a high-quality rice pan-genome, which provided a basis for further study of genes.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.905982