Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs

Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by...

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Bibliographic Details
Published in:Stem cells international 2021, Vol.2021, p.1-17
Main Authors: Zhang, Lifang, Li, Xinping, Shi, Congying, Ran, Gaoying, Peng, Yuting, Zeng, Shuguang, He, Yan
Format: Article
Language:English
Subjects:
Angiogenesis
Biocompatibility
Bones
CD34 antigen
Cell growth
Cells (biology)
Chitosan
Comparative analysis
Composite materials
Cord blood
Crosslinked polymers
Crosslinking
Cytotoxicity
Ethanol
Ethylenediaminetetraacetic acid
Gelatinase B
Genipin
Graphene
Hemopoiesis
Hydrogels
Mechanical properties
Nanomaterials
Progenitor cells
Scaffolds
Scanning electron microscopy
Scientific equipment and supplies industry
SDF-1 protein
Spectrum analysis
Tissue engineering
Toxicity
Transplants & implants
Umbilical cord
Vascular endothelial growth factor
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Summary:Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs.
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2021/5594370