Raman Spectroscopy as Noninvasive Method of Diagnosis of Pediatric Onset Inflammatory Bowel Disease

We propose here a spectroscopic method to diagnose and differentiate inflammatory bowel diseases (IBD), such as ulcerative colitis (UC) and Crohn’s disease (CD) with pediatric onset, in a complete noninvasive way without performing any duodenal biopsy. In particular, the Raman technique was applied...

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Bibliographic Details
Published in:Applied sciences 2020-10, Vol.10 (19), p.6974
Main Authors: Acri, Giuseppe, Venuti, Valentina, Costa, Stefano, Testagrossa, Barbara, Pellegrino, Salvatore, Crupi, Vincenza, Majolino, Domenico
Format: Article
Language:English
Subjects:
Biopsy
Children
Chronic illnesses
Crohn's disease
Crosslinking
Curve fitting
Diagnosis
Diagnosis, Noninvasive
Diagnostic tests
Diseases
Feces
Gastroenterology
Inflammation
Inflammatory bowel disease
Inflammatory bowel diseases
Intestine
Investigations
Lasers
Methods
Neutrophils
Pediatrics
Proteins
Raman spectroscopy
ROC curve
Software
Spectral signatures
Statistical analysis
Statistical methods
Ulcerative colitis
Vibrations
Working groups
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Summary:We propose here a spectroscopic method to diagnose and differentiate inflammatory bowel diseases (IBD), such as ulcerative colitis (UC) and Crohn’s disease (CD) with pediatric onset, in a complete noninvasive way without performing any duodenal biopsy. In particular, the Raman technique was applied to proteic extract from fecal samples in order to achieve information about molecular vibrations that can potentially furnish spectral signatures of cellular modifications occurring as a consequence of specific pathologic conditions. The attention was focused on the investigation of the amide I region, quantitatively accounting the spectral changes in the secondary structures by applying deconvolution and curve-fitting. Inflammation is found to give rise to a significant increasing of the nonreducible (trivalent)/reducible (divalent) cross-linking ratio R of the protein network. This parameter revealed an excellent marker in order to distinguish IBD subjects from non-IBD ones, and, among IBD patients, to differentiate between UC and CD. The proposed methodology was validated by statistical analysis using the receiver operating characteristic (ROC) curve.
ISSN:2076-3417
2076-3417
DOI:10.3390/app10196974