Synthesis and characterization of ethanolic extract of nutgalls of the plant Quercus infectoria silver nitrate nanoparticles incorporated chitosan based scaffold, and their in-silico study for wound healing activity

• Published in: Results in surfaces and interfaces 2025-01, Vol.18, p.100379, Article 100379
• Main Authors: Sahu, Madhusmita, Kamakshi, Sahoo, Jyotirmaya, Swain, Sudhansu Ranjan, Gupta, Sakshi, Fatma, Nishat, Mehara, Geetanjali
• Format: Article
• Language: English
• Subjects: Chitosan based scaffold; Docking score; Insilco study; LC-MS; Quercus infectoria; Silver nanoparticles; Total flavonoid content; Total phenol content
• ISSN: 2666-8459; 2666-8459
• DOI: 10.1016/j.rsurfi.2024.100379

Quercus infectoria is a popular medicinal plant used in many parts of the world as a treatment for various health conditions. The main components found in the galls of Quercus infectoria are tannin (50–70%) and small amounts of free Gallic acid and ellagic acid. The plant nut gall powder was extracted with 95% ethanol with ratio of 1:5 at room temperature for 7 days by a maceration process. Then it was filtered and evaporated on a rotary evaporator at 600 C until the mixture was completely dried. Various physicochemical properties were monitored. LC-MS study revealed the presence of various phytocomponents.Ten grams of pre-prepared plant extract is reconstituted to create a concentrated stock solution. The mixture is heated at 80 °C for 2 h with constant stirring and cooled to room temperature. Observation of a color change to dark brown indicates the formation of AgNPs. The gelled hydrogels prepared were freeze-dried to remove moisture, yielding porous scaffolds. The AgNPs synthesized using the pre-prepared plant extract showed a characteristic surface Plasmon resonance peak, which confirmed the successful formation of nanoparticles. The SEM image further shows a heterogeneous assortment of nanoparticles incorporated in chitosan based scaffold with different shapes and sizes, with more elongated shapes while others are more spherical. Scale bar in figure indicates 50 μm references for size comparison. Analysis shows composition of silver nanoparticles with significant amounts of carbon and oxygen. This composition is consistent with silver nanoparticles synthesized using plant extracts. The compounds named 4,4'-(9H-Fluorene-9,9-diyl)diphenol have higher binding affinity against 5XBD receptor with −8.9 kcal/mol and compound 3,4-Dihydroxyphenylglycol have higher affinity against 6Y8M receptor of −8.7 kcal/mol, which is better than the standard drug Silver Sulfadiazine. Moreover this shows the potent activity of proposed compounds against wound healing.