High levels of bisphenol A among infertile men can impair spermatogenesis by oxidative stress and elevated levels of microRNA-337

• Published in: Beni-Suef University journal of basic and applied sciences 2024-10, Vol.13 (1), p.103-8, Article 103
• Main Authors: Ahmad, Manar A., Ismail, Nashaat Nabil, Ghaleb, Sherien S., Hassan, Dalia Abd Elwhab, Al-Fakharany, Reham S., Ghoneim, Mohammed M., Ahmed, Amany M., Lotfy, Amr Mohamed, AboSief, Ahmed Fathy Mohamed
• Format: Article
• Language: English
• Subjects: Azoospermia; Bisphenol A; Chi-square test; Drug abuse; Ethics; Gene expression; Infertility; Lipid peroxidation; Lipids; Males; Malondialdehyde; Medical history; Medicine; Medicine & Public Health; MicroRNA-337; MicroRNAs; miRNA; Motility; Oligozoospermia; Oxidative stress; Ribonucleic acid; RNA; Rural areas; Semen; Sperm; Spermatogenesis; Statistical analysis; Testosterone; Urine
• ISSN: 2314-8543; 2314-8535; 2314-8543
• DOI: 10.1186/s43088-024-00559-w

Background Studies have shown that Bisphenol A may interfere with the process of spermatogenesis and result in a decrease in the quality of semen. Nevertheless, the fundamental processes remain unclear. This study was done to investigate the connections between exposure to Bisphenol A, spermatogenesis with microRNA-337, and malondialdehyde in infertile men. Methods This study was a case–control study on 73 participants. Infertile group (1a): azoospermia ( n  = 16), infertile group (1b): oligozoospermia ( n  = 22), and control group (2): normospermic ( n  = 35) were enrolled in this study. Full history, local examination, semen analysis, and urine and blood samples were taken from all participants. Urinary Bisphenol A, malondialdehyde, and serum microRNA-337 were measured. Results The mean Bisphenol A level in azoospermia group shows statistically significant increase comparing to fertile control group. The mean microRNA-337 level in oligozoospermia and azoospermia group shows statistically significant increase comparing to fertile controls. The mean malondialdehyde level in infertility groups shows statistically significant increase comparing to fertile control group. No linear correlations were recorded between Bisphenol A levels with semen quality parameters, hormonal profile, and microRNA-337. Conclusion While there is no significant change in the levels of Bisphenol A between normal fertile males and infertile males with oligozospermia, a significant elevation in the BPA level was observed in infertile males with azoospermia. A significant upregulation of the miRNA-337 gene expression in infertile males either oligozospermia or azoospermia was also observed. In addition, lipid peroxidation as evident by the significant elevation of MDA levels was marked among infertile patients.